|
| Status |
Public on Sep 14, 2023 |
| Title |
14-OB-Input_S14 |
| Sample type |
SRA |
| |
|
| Source name |
OB
|
| Organism |
Mus musculus |
| Characteristics |
tissue: OB
genotype: WT
chip antibody: none
|
| Growth protocol |
No culturing
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tissue was fixed with 1% formaldehyde for 10 minutes, quenched with 150mM Glycine, sonicated on a Qsonica Q800 for 30 minutes on a 10sec on-off schedule at 15-20W per tube
We used the Rubicon genomics Thruplex DNA-seq kit for library preparation
Three pools, 48 barcodeseach
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Aligning
Peak calling
Diffbind (details in methods)
Assembly: mm10
Supplementary files format and content: Count table output from DiffBind in Rdata format.
|
| |
|
| Submission date |
Aug 03, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Stefan Blankvoort |
| E-mail(s) |
stefan.blankvoort@ntnu.no
|
| Organization name |
NTNU
|
| Department |
Kavli Institute for systems Neuroscience
|
| Lab |
Kentros
|
| Street address |
Olav Kyrresgt 9
|
| City |
Trondheim |
| ZIP/Postal code |
7030 |
| Country |
Norway |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE240042 |
A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents |
|
| Relations |
| BioSample |
SAMN36830120 |
| SRA |
SRX21242473 |
