|
Status |
Public on Sep 14, 2023 |
Title |
A1-VisCtx-K27Ac |
Sample type |
SRA |
|
|
Source name |
VisCtx
|
Organism |
Mus musculus |
Characteristics |
tissue: VisCtx genotype: WT chip antibody: K27Ac (Diagenode H3K27Ac Rabbit polyclonal, Cat# C15410196, lot# A1723-0041D)
|
Growth protocol |
No culturing
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Tissue was fixed with 1% formaldehyde for 10 minutes, quenched with 150mM Glycine, sonicated on a Qsonica Q800 for 30 minutes on a 10sec on-off schedule at 15-20W per tube We used the Rubicon genomics Thruplex DNA-seq kit for library preparation Three pools, 48 barcodeseach
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Aligning Peak calling Diffbind (details in methods) Assembly: mm10 Supplementary files format and content: Count table output from DiffBind in Rdata format.
|
|
|
Submission date |
Aug 03, 2023 |
Last update date |
Sep 14, 2023 |
Contact name |
Stefan Blankvoort |
E-mail(s) |
stefan.blankvoort@ntnu.no
|
Organization name |
NTNU
|
Department |
Kavli Institute for systems Neuroscience
|
Lab |
Kentros
|
Street address |
Olav Kyrresgt 9
|
City |
Trondheim |
ZIP/Postal code |
7030 |
Country |
Norway |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE240042 |
A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents |
|
Relations |
BioSample |
SAMN36830147 |
SRA |
SRX21242442 |