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Sample GSM7680440 Query DataSets for GSM7680440
Status Public on Sep 14, 2023
Title A1-VisCtx-K27Ac
Sample type SRA
 
Source name VisCtx
Organism Mus musculus
Characteristics tissue: VisCtx
genotype: WT
chip antibody: K27Ac (Diagenode H3K27Ac Rabbit polyclonal, Cat# C15410196, lot# A1723-0041D)
Growth protocol No culturing
Extracted molecule genomic DNA
Extraction protocol Tissue was fixed with 1% formaldehyde for 10 minutes, quenched with 150mM Glycine, sonicated on a Qsonica Q800 for 30 minutes on a 10sec on-off schedule at 15-20W per tube
We used the Rubicon genomics Thruplex DNA-seq kit for library preparation
Three pools, 48 barcodeseach
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing Aligning
Peak calling
Diffbind (details in methods)
Assembly: mm10
Supplementary files format and content: Count table output from DiffBind in Rdata format.
 
Submission date Aug 03, 2023
Last update date Sep 14, 2023
Contact name Stefan Blankvoort
E-mail(s) stefan.blankvoort@ntnu.no
Organization name NTNU
Department Kavli Institute for systems Neuroscience
Lab Kentros
Street address Olav Kyrresgt 9
City Trondheim
ZIP/Postal code 7030
Country Norway
 
Platform ID GPL13112
Series (1)
GSE240042 A novel viral tool designed with enhancer-driven gene expression (EDGE) technology allows specific labelling of cell types in wild type rodents
Relations
BioSample SAMN36830147
SRA SRX21242442

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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