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Sample GSM7677398 Query DataSets for GSM7677398
Status Public on Aug 07, 2023
Title hESCs cells, D60, replicate 2
Sample type SRA
 
Source name hESCs
Organism Homo sapiens
Characteristics cell line: hESCs
cell type: Human embryonic stem cells
time: Day60
Treatment protocol To initiate differentiation, the E8 medium was changed to RPMI/B27 medium lacking insulin. CHIR99021 (6 μM; Selleckchem) was added to the cells from days 0 to 2, followed by the addition of IWR-1 (10 μM; Selleckchem) from days 3 to 5 for H7 cells. After day 7, cultures were maintained in RPMI/B27 medium containing insulin (Thermo Scientific), which was changed twice a week.
Growth protocol Undifferentiated H7 ESCs were maintained on Matrigel (Thermo Scientific)-coated surfaces at 37°C and 5% CO2 in E8 medium plus supplements (Thermo Scientific)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an RNeasy micro kit (Qiagen), according to the manufacturer’s protocol.
cDNA libraries were prepared by KAPA Stranded mRNA-Seq Kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Data processing Raw reads were aligned to hs37d5 using Tophat
Read count extraction and normalization were performed using htseq-count and Deseq-2
Assembly: hs37d5
Supplementary files format and content: tab-delimited files include raw counts for each sample
Supplementary files format and content: tab-delimited files include FPKMs for each sample
 
Submission date Aug 02, 2023
Last update date Aug 07, 2023
Contact name Ellen Ngar-Yun Poon
Organization name The Chinese University of Hong Kong
Department School of Biomedical Sciences
Street address Shatin
City Hong Kong
State/province Hong Kong
ZIP/Postal code 999077
Country China
 
Platform ID GPL18460
Series (1)
GSE239918 Dynamic changes of mRNA expression during development of human embryonic stem cells derived cardiomyocytes
Relations
BioSample SAMN36811681
SRA SRX21228792

Supplementary file Size Download File type/resource
GSM7677398_D60_2_fpkm.txt.gz 257.3 Kb (ftp)(http) TXT
GSM7677398_D60_2_rawCount.txt.gz 204.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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