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Sample GSM7673462 Query DataSets for GSM7673462
Status Public on Jan 25, 2024
Title WT, L21, 4
Sample type SRA
 
Source name heart
Organism Mus musculus
Characteristics developmental stage: E13.5
tissue: heart
genotype: WT
treatment: L21
Treatment protocol Pregnant mice were treated daily by oral gavage with 30mg/kg of L21 or Iso-L21 from embryonic day 5.5 to E13.5.
Extracted molecule total RNA
Extraction protocol E13.5 frozen embryonic hearts were placed in lysis buffer and homogenized using a cordless Pellet pestle (Sigma-Aldrich) followed by RNA extraction using RNAeasy micro kit (Qiagen)
polyA enriched libraries usingNEBNext®Ultra™II Directional RNA Library Prep Kit for Illumina according to manufacturer’s instructions. An average 25 million pair end reads per library (PE100) were generated on a NovaSeq6000 (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing nf-core Nextflow RNAseq pipeline, revision 3.10.1 (https://github.com/nf-core/rnaseq), with STAR aligner and RSEM quantification - all other parameers as default
Assembly: GRCm38, release 95
Supplementary files format and content: tab-delimited text file containing expected counts, TPM, FPKM per gene detected
 
Submission date Aug 01, 2023
Last update date Jan 25, 2024
Contact name Victor Tybulewicz
E-mail(s) Victor.T@crick.ac.uk
Organization name Francis Crick Institute
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
 
Platform ID GPL24247
Series (2)
GSE196447 Down Syndrome
GSE239798 Effect of Dyrk1a inhibition on the transcriptome of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using bulk RNAseq
Relations
BioSample SAMN36795294
SRA SRX21218685

Supplementary file Size Download File type/resource
GSM7673462_LAN5770A6.genes.results.gz 1.1 Mb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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