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Sample GSM7655992 Query DataSets for GSM7655992
Status Public on May 22, 2024
Title APPNL-G-F mouse with exercise, hippocampus, snRNAseq
Sample type SRA
 
Source name Hippocampus
Organism Mus musculus
Characteristics tissue: Hippocampus
Sex: Female
genotype: APPNL-G-F
treatment: Exercise
Treatment protocol Mice were housed in cages with running wheel for voluntary exercise for 10 weeks.
Growth protocol Eight-week old female WT and NLGF C57BL/6J mice were fed ab lib with normal chow diet
Extracted molecule total RNA
Extraction protocol Hippocampus was isolated immediately on ice, washed with cold D-PBS, and snap-frozen with liquid nitrogen. The Frankenstein' protocol was adopted to isolate the nuclei from frozen tissues (https://www.protocols.io/view/frankenstein-protocol-for-nuclei-isolation-from-f-5jyl8nx98l2w/v2).
Chromium NextGEM SingleCell 3-_GeneExpression_v3.1_DualIndex
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v3.1.0
Assembly: mm10
Supplementary files format and content: Tab-separated bacode files
Supplementary files format and content: Tab-separated feature files
Supplementary files format and content: Tab-separated matrix files
 
Submission date Jul 20, 2023
Last update date May 22, 2024
Contact name Zheng Sun
E-mail(s) Zheng.Sun@bcm.edu
Organization name Baylor College of Medicine
Street address 1329 Moursund St
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL24247
Series (2)
GSE237885 Gene expression profile at single nuclei level of cells from mouse hippocampus
GSE237925 Single-nucleus multi-omics dissection of physical exercise effects on neurocognition
Relations
BioSample SAMN36661643
SRA SRX21114035

Supplementary file Size Download File type/resource
GSM7655992_APP_EX_barcodes.tsv.gz 47.1 Kb (ftp)(http) TSV
GSM7655992_APP_EX_features.tsv.gz 284.1 Kb (ftp)(http) TSV
GSM7655992_APP_EX_matrix.mtx.gz 95.6 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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