|
Status |
Public on Nov 03, 2011 |
Title |
ES cell 36 hours in low-adhesion dishes w/o LIF 60 s CYH (100 ug/ml) ribo_mesc_nolif Illumina HiSeq |
Sample type |
SRA |
|
|
Source name |
ES cell
|
Organism |
Mus musculus |
Characteristics |
treatment: ES cell 36 hours in low-adhesion dishes w/o LIF 60 s CYH (100 ug/ml) genetic background: 129/Ola cell line: E14
|
Treatment protocol |
60 s cycloheximide (100 ug/ml)
|
Growth protocol |
36 hours in low-adhesion dishes without LIF
|
Extracted molecule |
total RNA |
Extraction protocol |
Rnase I footprinting, ribosome pelleting, RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
other |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
Ribosome Profiling
|
Data processing |
Alignment to transcriptome (mm9) using 25nt seed and extension to linker sequence
|
|
|
Submission date |
Jul 21, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Nicholas T Ingolia |
E-mail(s) |
nick@ingolia.org
|
Phone |
410 246 3025
|
Organization name |
Carnegie Institution
|
Department |
Embryology
|
Lab |
Ingolia
|
Street address |
3520 San Martin Drive
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21218 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE30839 |
RibosomeĀ ProfilingĀ of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes |
|
Relations |
SRA |
SRX084823 |
BioSample |
SAMN00672698 |