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Sample GSM765291 Query DataSets for GSM765291
Status Public on Nov 03, 2011
Title embryoid body 8d in low-adhesion dishes w/o LIF 60 s CYH (100 ug/ml) ribo_eb Illumina HiSeq
Sample type SRA
Source name embryoid body
Organism Mus musculus
Characteristics treatment: embryoid body 8d in low-adhesion dishes w/o LIF 60 s CYH (100 ug/ml)
genetic background: 129/Ola
cell line: E14
Treatment protocol 60 s cycloheximide (100 ug/ml)
Growth protocol 8 days in low-adhesion dishes without LIF
Extracted molecule total RNA
Extraction protocol Rnase I footprinting, ribosome pelleting, RNA extraction, enzymatic polyadenylation, dT-primed reverse transcription, cDNA circularization, PCR amplification
Library strategy RNA-Seq
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
Description Ribosome Profiling
Data processing Alignment to transcriptome (mm9) using 25nt seed and extension to linker sequence
Submission date Jul 21, 2011
Last update date May 15, 2019
Contact name Nicholas T Ingolia
Phone 410 246 3025
Organization name Carnegie Institution
Department Embryology
Lab Ingolia
Street address 3520 San Martin Drive
City Baltimore
State/province MD
ZIP/Postal code 21218
Country USA
Platform ID GPL13112
Series (1)
GSE30839 RibosomeĀ ProfilingĀ of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes
SRA SRX084814
BioSample SAMN00672689

Supplementary file Size Download File type/resource
GSM765291_ribo_eb_110414l6_trxs_norrna.align.txt.gz 2.7 Gb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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