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Sample GSM76189 Query DataSets for GSM76189
Status Public on Jun 30, 2006
Title Noggin_stg11.5_clutch4
Sample type RNA
 
Source name Ventral half of stg11.5 gastrula, injected with noggin
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 4;
Tissue: Ventral half of gastrula embryo;
Injection: noggin and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with noggin plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
 
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
E-mail(s) alhufton@stanford.edu
Phone 650 736 0278
URL http://www.stanford.edu/group/bakerlab/
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
ID_REF
VALUE RMA expression, not log'ed

Data table
ID_REF VALUE
AFFX-BioB-3_at 117.8572746
AFFX-BioB-5_at 144.9032356
AFFX-BioB-M_at 163.198049
AFFX-BioC-3_at 370.3198312
AFFX-BioC-5_at 354.3759777
AFFX-BioDn-3_at 1716.689018
AFFX-BioDn-5_at 539.468533
AFFX-CreX-3_at 3512.708147
AFFX-CreX-5_at 3052.039672
AFFX-DapX-3_at 9.680442529
AFFX-DapX-5_at 9.694261505
AFFX-DapX-M_at 12.54254318
AFFX-LysX-3_at 11.00904943
AFFX-LysX-5_at 8.211495537
AFFX-LysX-M_at 15.28366886
AFFX-PheX-3_at 63.69944952
AFFX-PheX-5_at 9.383043801
AFFX-PheX-M_at 9.881054407
AFFX-ThrX-3_at 34.54454489
AFFX-ThrX-5_at 15.94646789

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM76189.CEL.gz 3.4 Mb (ftp)(http) CEL

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