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Sample GSM76188 Query DataSets for GSM76188
Status Public on Jun 30, 2006
Title Ventral_stg11.5_clutch3
Sample type RNA
Source name Ventral half of stg11.5 gastrula
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 3;
Tissue: Ventral half of gastrula embryo;
Injection: eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description The Dorsal_stg11.5_clutch3 sample was derived from the same 10 eGFP injected embryos as this sample.
Data processing RMA method, values in the GEO database have not been log transformed.
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
Phone 650 736 0278
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
VALUE RMA expression, not log'ed

Data table
AFFX-BioB-3_at 64.0177914
AFFX-BioB-5_at 97.67781342
AFFX-BioB-M_at 83.62596218
AFFX-BioC-3_at 211.7799206
AFFX-BioC-5_at 248.8069543
AFFX-BioDn-3_at 1157.668918
AFFX-BioDn-5_at 365.2670989
AFFX-CreX-3_at 2491.983844
AFFX-CreX-5_at 2664.797148
AFFX-DapX-3_at 10.53820321
AFFX-DapX-5_at 10.07307321
AFFX-DapX-M_at 15.85604437
AFFX-LysX-3_at 11.7399959
AFFX-LysX-5_at 8.658445302
AFFX-LysX-M_at 13.88274547
AFFX-PheX-3_at 75.14748935
AFFX-PheX-5_at 10.75412321
AFFX-PheX-M_at 10.46184213
AFFX-ThrX-3_at 34.59937327
AFFX-ThrX-5_at 13.98413391

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM76188.CEL.gz 2.8 Mb (ftp)(http) CEL

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