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Sample GSM76184 Query DataSets for GSM76184
Status Public on Jun 30, 2006
Title Dkk-1_stg11.5_clutch3
Sample type RNA
 
Source name Ventral half of stg11.5 gastrula, injected with dkk-1
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 3;
Tissue: Ventral half of gastrula embryo;
Injection: dkk-1 and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with dkk-1 plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
 
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
E-mail(s) alhufton@stanford.edu
Phone 650 736 0278
URL http://www.stanford.edu/group/bakerlab/
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
ID_REF
VALUE RMA expression, not log'ed

Data table
ID_REF VALUE
AFFX-BioB-3_at 47.1432724
AFFX-BioB-5_at 63.05592354
AFFX-BioB-M_at 68.86151422
AFFX-BioC-3_at 143.8791768
AFFX-BioC-5_at 152.9132911
AFFX-BioDn-3_at 945.6208266
AFFX-BioDn-5_at 287.1629872
AFFX-CreX-3_at 2283.287797
AFFX-CreX-5_at 2383.344855
AFFX-DapX-3_at 9.187962701
AFFX-DapX-5_at 11.16580995
AFFX-DapX-M_at 13.1563191
AFFX-LysX-3_at 11.16574029
AFFX-LysX-5_at 7.249768864
AFFX-LysX-M_at 14.81615303
AFFX-PheX-3_at 79.03769672
AFFX-PheX-5_at 9.068783258
AFFX-PheX-M_at 11.50025543
AFFX-ThrX-3_at 32.08339705
AFFX-ThrX-5_at 16.48526302

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM76184.CEL.gz 2.9 Mb (ftp)(http) CEL

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