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Sample GSM76182 Query DataSets for GSM76182
Status Public on Jun 30, 2006
Title Noggin+Dkk-1_stg11.5_clutch3
Sample type RNA
Source name Ventral half of stg11.5 gastrula, injected with noggin and dkk-1
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 3;
Tissue: Ventral half of gastrula embryo;
Injection: noggin, dkk-1, and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with noggin and dkk-1 plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
Phone 650 736 0278
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
VALUE RMA expression, not log'ed

Data table
AFFX-BioB-3_at 42.78552832
AFFX-BioB-5_at 46.7976766
AFFX-BioB-M_at 53.18689828
AFFX-BioC-3_at 151.8568313
AFFX-BioC-5_at 149.8155952
AFFX-BioDn-3_at 879.5257463
AFFX-BioDn-5_at 337.3433705
AFFX-CreX-3_at 2480.465197
AFFX-CreX-5_at 2429.027318
AFFX-DapX-3_at 8.289396088
AFFX-DapX-5_at 9.636304618
AFFX-DapX-M_at 12.33436894
AFFX-LysX-3_at 10.72654086
AFFX-LysX-5_at 8.187823481
AFFX-LysX-M_at 14.90828088
AFFX-PheX-3_at 63.70412992
AFFX-PheX-5_at 9.795302183
AFFX-PheX-M_at 10.30685303
AFFX-ThrX-3_at 36.04694274
AFFX-ThrX-5_at 17.64364827

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM76182.CEL.gz 3.0 Mb (ftp)(http) CEL

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