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Sample GSM76172 Query DataSets for GSM76172
Status Public on Jun 30, 2006
Title Dkk-1_stg10_clutch2
Sample type RNA
 
Source name Ventral half of stg10 gastrula, injected with dkk-1
Organism Xenopus laevis
Characteristics Stage: 10;
Clutch: 1;
Tissue: Ventral half of gastrula embryo;
Injection: dkk-1 and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with dkk-1 plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 10 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
 
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
E-mail(s) alhufton@stanford.edu
Phone 650 736 0278
URL http://www.stanford.edu/group/bakerlab/
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
ID_REF
VALUE RMA expression, not log'ed

Data table
ID_REF VALUE
AFFX-BioB-3_at 147.4810722
AFFX-BioB-5_at 171.822243
AFFX-BioB-M_at 199.698405
AFFX-BioC-3_at 278.6499337
AFFX-BioC-5_at 326.3920257
AFFX-BioDn-3_at 1450.486346
AFFX-BioDn-5_at 254.9732346
AFFX-CreX-3_at 3727.010065
AFFX-CreX-5_at 3016.412462
AFFX-DapX-3_at 9.245843866
AFFX-DapX-5_at 10.79192259
AFFX-DapX-M_at 13.14733969
AFFX-LysX-3_at 12.36344592
AFFX-LysX-5_at 7.890036641
AFFX-LysX-M_at 13.76156842
AFFX-PheX-3_at 70.2493027
AFFX-PheX-5_at 9.945866042
AFFX-PheX-M_at 11.44972466
AFFX-ThrX-3_at 36.24303834
AFFX-ThrX-5_at 17.4613476

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.




Supplementary file Size Download File type/resource
GSM76172.CEL.gz 3.3 Mb (ftp)(http) CEL

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