|
| Status |
Public on Feb 28, 2012 |
| Title |
A1-2_DEX#1 |
| Sample type |
RNA |
| |
|
| Source name |
A1-2_DEX_8h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A1-2 breast cancer cells
agent: dexamethasone
|
| Treatment protocol |
100 nM dexamethasone or 0.1% ethanol vehicle for 8 hours.
|
| Growth protocol |
48 hours in DMEM supplemented with 5% charcoal dextran treated FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Plus with on column DNase digestion per manufactures protocol
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
100 nm Dexamethasone treatment 8h
251485051236_1_2
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
Feb 28, 2012 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE30592 |
A1-2 expression analysis of GR activation |
|
