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Sample GSM75884 Query DataSets for GSM75884
Status Public on May 01, 2006
Title coronary smooth muscle cells nebivolol replicate 3
Sample type RNA
Source name coronary smooth muscle cells (hcaSMC), treated with nebivolol for 72 hours
Organism Homo sapiens
Characteristics nebivolol treated muscle cells
Extracted molecule total RNA
Extraction protocol total RNA of human coronary artery smooth muscle (hcaSMC) cultivated in SMGM-2-MV bulletkit medium were isolated after incubation with nebivolol for 72 hours using Trizol and purified with RNeasy spin columns.
Label biotin
Label protocol Approximately 7 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description human coronary artery smooth muscle Cells, treated with nebivolol, replicate 3
Data processing Affymetrix Microarray Suite version 5.0
Submission date Sep 23, 2005
Last update date Sep 23, 2005
Contact name Bernhard R. Brehm
Phone +4970712982711
Fax +497071360245
Organization name University of Tuebingen
Street address Otfried-Mueller-Str. 10
City Tuebingen
ZIP/Postal code 72076
Country Germany
Platform ID GPL96
Series (1)
GSE3356 Effects of metoprolol and nebivolol on gene expresion in human coronary smooth muscle cells

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 50.7 M 0.05023
AFFX-BioB-M_at 44.8 P 0.00844
AFFX-BioB-3_at 31.2 A 0.08948
AFFX-BioC-5_at 139.8 P 0.0011
AFFX-BioC-3_at 96.1 P 0.00141
AFFX-BioDn-5_at 143.7 P 0.00097
AFFX-BioDn-3_at 1105.2 P 0.00075
AFFX-CreX-5_at 1288.9 P 0.00004
AFFX-CreX-3_at 2259.3 P 0.00004
AFFX-DapX-5_at 4.9 A 0.35445
AFFX-DapX-M_at 11.8 A 0.2508
AFFX-DapX-3_at 2 A 0.81487
AFFX-LysX-5_at 2.7 A 0.64555
AFFX-LysX-M_at 12.1 A 0.48511
AFFX-LysX-3_at 4.3 A 0.48511
AFFX-PheX-5_at 1 A 0.93707
AFFX-PheX-M_at 0.7 A 0.94977
AFFX-PheX-3_at 16.6 A 0.54459
AFFX-ThrX-5_at 3.5 A 0.65934
AFFX-ThrX-M_at 7.5 A 0.39692

Total number of rows: 22283

Table truncated, full table size 562 Kbytes.

Supplementary file Size Download File type/resource
GSM75884.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM75884.EXP.gz 563 b (ftp)(http) EXP

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