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Sample GSM75877 Query DataSets for GSM75877
Status Public on May 01, 2006
Title coronary smooth muscle cells control replicate 2
Sample type RNA
Source name coronary smooth muscle cells (hcaSMC), treated with control medium for 72 hours
Organism Homo sapiens
Characteristics control, untreated muscle cells
Extracted molecule total RNA
Extraction protocol total RNA of human coronary artery smooth muscle (hcaSMC) cultivated in SMGM-2-MV bulletkit medium were isolated after incubation with control medium for 72 hours using Trizol and purified with RNeasy spin columns.
Label biotin
Label protocol Approximately 7 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description human coronary artery smooth muscle Cells, untreated control, replicate 2
Data processing Affymetrix Microarray Suite version 5.0
Submission date Sep 23, 2005
Last update date Sep 23, 2005
Contact name Bernhard R. Brehm
Phone +4970712982711
Fax +497071360245
Organization name University of Tuebingen
Street address Otfried-Mueller-Str. 10
City Tuebingen
ZIP/Postal code 72076
Country Germany
Platform ID GPL96
Series (1)
GSE3356 Effects of metoprolol and nebivolol on gene expresion in human coronary smooth muscle cells

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 187.8 P 0.0018
AFFX-BioB-M_at 402 P 0.00009
AFFX-BioB-3_at 114.2 P 0.00075
AFFX-BioC-5_at 621 P 0.00013
AFFX-BioC-3_at 574.2 P 0.00008
AFFX-BioDn-5_at 639.9 P 0.00004
AFFX-BioDn-3_at 3158.3 P 0.00005
AFFX-CreX-5_at 5476.8 P 0.00004
AFFX-CreX-3_at 7087.5 P 0.00004
AFFX-DapX-5_at 10.9 A 0.30061
AFFX-DapX-M_at 29.2 A 0.23906
AFFX-DapX-3_at 3.9 A 0.85206
AFFX-LysX-5_at 1.9 A 0.69939
AFFX-LysX-M_at 30.7 A 0.30061
AFFX-LysX-3_at 6.9 A 0.39692
AFFX-PheX-5_at 1.3 A 0.84327
AFFX-PheX-M_at 3.1 A 0.922
AFFX-PheX-3_at 5.1 A 0.98172
AFFX-ThrX-5_at 5 A 0.72485
AFFX-ThrX-M_at 4.4 A 0.73717

Total number of rows: 22283

Table truncated, full table size 562 Kbytes.

Supplementary file Size Download File type/resource
GSM75877.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM75877.EXP.gz 558 b (ftp)(http) EXP

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