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Sample GSM75876 Query DataSets for GSM75876
Status Public on May 01, 2006
Title coronary smooth muscle cells control replicate 1
Sample type RNA
 
Source name coronary smooth muscle cells (hcaSMC), treated with control medium for 72 hours
Organism Homo sapiens
Characteristics control, untreated muscle cells
Extracted molecule total RNA
Extraction protocol total RNA of human coronary artery smooth muscle (hcaSMC) cultivated in SMGM-2-MV bulletkit medium were isolated after incubation with control medium for 72 hours using Trizol and purified with RNeasy spin columns.
Label biotin
Label protocol Approximately 7 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description human coronary artery smooth muscle Cells, untreated control, replicate 1
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 23, 2005
Last update date Sep 23, 2005
Contact name Bernhard R. Brehm
E-mail(s) bernhard.brehm@onlinehome.de
Phone +4970712982711
Fax +497071360245
Organization name University of Tuebingen
Street address Otfried-Mueller-Str. 10
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platform ID GPL96
Series (1)
GSE3356 Effects of metoprolol and nebivolol on gene expresion in human coronary smooth muscle cells

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 149.3 P 0.00448
AFFX-BioB-M_at 342 P 0.0002
AFFX-BioB-3_at 106.6 P 0.005
AFFX-BioC-5_at 568.2 P 0.00009
AFFX-BioC-3_at 425.9 P 0.00006
AFFX-BioDn-5_at 483 P 0.00005
AFFX-BioDn-3_at 3205.5 P 0.00008
AFFX-CreX-5_at 4493.6 P 0.00004
AFFX-CreX-3_at 6189.2 P 0.00004
AFFX-DapX-5_at 2.2 A 0.67292
AFFX-DapX-M_at 25.3 A 0.14794
AFFX-DapX-3_at 14.8 A 0.48511
AFFX-LysX-5_at 3.9 A 0.71226
AFFX-LysX-M_at 5.9 A 0.68628
AFFX-LysX-3_at 4.8 A 0.45541
AFFX-PheX-5_at 2.8 A 0.78348
AFFX-PheX-M_at 2.6 A 0.91052
AFFX-PheX-3_at 20.9 A 0.60308
AFFX-ThrX-5_at 3.9 A 0.89783
AFFX-ThrX-M_at 8.4 A 0.48511

Total number of rows: 22283

Table truncated, full table size 562 Kbytes.




Supplementary file Size Download File type/resource
GSM75876.CEL.gz 3.3 Mb (ftp)(http) CEL
GSM75876.EXP.gz 546 b (ftp)(http) EXP

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