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Sample GSM753 Query DataSets for GSM753
Status Public on Nov 28, 2001
Title SAGE_SciencePark_MCF7_estradiol_3h
Sample type SAGE
Anchor NlaIII
Tag Count 60162
Tag Length 10
 
Source name Breast Carcinoma - Pleaural effusion
Organism Homo sapiens
Extracted molecule total RNA
 
Description MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) - This SAGE library represents the 3h time-point of E2 treatment
Sex: Female
Organ: Breast
Cell_type: epithelial
Cell_line: MCF7
Lab host: Top10 bacteria
Vector: pZero
Vector type: plasmid
R. Site 1: NlaIII
R. Site 2: BsmF1 as tagging enzyme
Sequences generated to date: 1838

This library represents a Cancer Genome Anatomy Project library, which was either produced through CGAP funding, or donated to CGAP.
The Cancer Genome Anatomy Project (CGAP: http://cgap.nci.nih.gov) is an interdisciplinary program established and administered by the National Cancer Institute (NCI: http://www.nci.nih.gov) to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell.
Keywords = mammary gland
Keywords = carcinoma
Keywords = cell line
Keywords = estrogen
Keywords = EST
 
Submission date Nov 28, 2001
Last update date May 28, 2005
Contact name C Marcelo Aldaz
E-mail(s) maldaz@odin.mdacc.tmc.edu
Phone 512-237-9530
URL http://sciencepark.mdanderson.org/ggeg
Organization name M.D. Anderson Cancer Center
Department Science Park Research Division, Carcinogenesis
Street address
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
 
Platform ID GPL4
Series (1)
GSE41 Effects of estrogen

Data table header descriptions
TAG Ten base SAGE tag,
COUNT Absolute tag count
TPM Tags per million, or (1000000*COUNT)/(Total tags)

Data table
TAG COUNT TPM
ACTTTTTCAA 762 12665.8
CCTCCAGCTA 656 10903.9
TTGGTCCTCT 554 9208.47
CCAGAACAGA 419 6964.53
CTGGGTTAAT 378 6283.04
AGGCTACGGA 357 5933.98
AAGACAGTGG 336 5584.92
AAGGTGGAGG 325 5402.08
CAAATGAGGA 274 4554.37
AATCCTGTGG 263 4371.53
AAGGAGATGG 258 4288.42
AGCACCTCCA 257 4271.8
ATAATTCTTT 256 4255.18
CACAAACGGT 253 4205.31
GCATAATAGG 251 4172.07
ACATCATCGA 248 4122.2
CAAACCATCC 247 4105.58
TACCATCAAT 241 4005.85
CCCGTCCGGA 240 3989.23
CTCATAAGGA 239 3972.61

Total number of rows: 17821

Table truncated, full table size 366 Kbytes.




Supplementary data files not provided

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