|
Status |
Public on Feb 16, 2012 |
Title |
P5_MUT2 |
Sample type |
genomic |
|
|
Source name |
human astrocytes
|
Organism |
Homo sapiens |
Characteristics |
tissue type: cell line cell type: astrocyte status: --
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The genomic DNA was extracted from snap-frozen sections from the breast cancer patients by using a DNeasy Tissue kit (Qiagen)
|
Label |
Biotin
|
Label protocol |
Standard Illumina protocol for Human Methylation27 DNA Analysis bead chip
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
Gene methylation data data for glioma cell line
|
Data processing |
data was normalized and analyzed using partek software and R packages. Specifically centered logit transformation was used for data normalization.
|
|
|
Submission date |
Jun 30, 2011 |
Last update date |
Feb 28, 2012 |
Contact name |
FANG FANG |
E-mail(s) |
fangf100088@gmail.com
|
Phone |
646-8882783
|
Fax |
646-8882595
|
Organization name |
Memorial Sloan-Kettering Cancer Center
|
Department |
The Human Oncology and Pathogenesis Program
|
Lab |
Timothy Chan
|
Street address |
1275 York Avenue
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE30338 |
Methylation analysis of 81 glioma clinical samples (49 CIMP+ and 32 CIMP-) and 53 cell line samples |
GSE30339 |
IDH1 Mutation is a Master Regulator of Epigenomic Remodeling and is Sufficient to Establish the Glioma Hypermethylator Phenotype |
|