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Status |
Public on Jan 17, 2024 |
Title |
CML Chronic Phase, Diagnosis, Lin-CD34+CD38-, CITEseq [CML5_38n] |
Sample type |
SRA |
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Source name |
bone marrow
|
Organism |
Homo sapiens |
Characteristics |
tissue: bone marrow cell type: hematopoietic stem and progenitor cells
|
Extracted molecule |
polyA RNA |
Extraction protocol |
FACS sorted Lin-CD34+ and Lin-CD34+CD38- cells are directly proceeded to single-cell droplet generation with 10X Genomics platform and further undergone library preparation. library preparation was done according to the Chromium Single Cell 3’ Reagent Kit v3.1 protocol
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Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
obtained sequence reads were processed using CellRanger (10X Genomics) pipeline The data was processed using Scarf. Assembly: hg38 Supplementary files format and content: Each tar file gives the counts in mtx format, the ADT counts, and the metadata for each cell including UMAP co-ordinates.
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Submission date |
Jun 30, 2023 |
Last update date |
Jan 17, 2024 |
Contact name |
Shamit Soneji |
Organization name |
BMC
|
Street address |
Solvegatan 19
|
City |
Lund |
ZIP/Postal code |
22184 |
Country |
Sweden |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE236233 |
Single cell multi-omics analysis of chronic myeloid leukemia links cellular heterogeneity to therapy response |
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