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Sample GSM7520170 Query DataSets for GSM7520170
Status Public on Jan 17, 2024
Title CML Chronic Phase, Diagnosis, Lin-CD34+CD38-, CITEseq [CML5_38n]
Sample type SRA
 
Source name bone marrow
Organism Homo sapiens
Characteristics tissue: bone marrow
cell type: hematopoietic stem and progenitor cells
Extracted molecule polyA RNA
Extraction protocol FACS sorted Lin-CD34+ and Lin-CD34+CD38- cells are directly proceeded to single-cell droplet generation with 10X Genomics platform and further undergone library preparation.
library preparation was done according to the Chromium Single Cell 3’ Reagent Kit v3.1 protocol
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing obtained sequence reads were processed using CellRanger (10X Genomics) pipeline
The data was processed using Scarf.
Assembly: hg38
Supplementary files format and content: Each tar file gives the counts in mtx format, the ADT counts, and the metadata for each cell including UMAP co-ordinates.
 
Submission date Jun 30, 2023
Last update date Jan 17, 2024
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
 
Platform ID GPL24676
Series (1)
GSE236233 Single cell multi-omics analysis of chronic myeloid leukemia links cellular heterogeneity to therapy response

Supplementary file Size Download File type/resource
GSM7520170_CML5_38n.tar.gz 5.4 Mb (ftp)(http) TAR
Processed data provided as supplementary file
Raw data not provided for this record

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