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Sample GSM7498398 Query DataSets for GSM7498398
Status Public on Mar 15, 2024
Title P14 T cells sorted from B16-OVA tumor treated with PBS, Replicate 2
Sample type SRA
Source name P14_T_cells
Organism Mus musculus
Characteristics cell type: P14_T_cells
strain: C57BL/6
Extracted molecule total RNA
Extraction protocol Single-cell suspensions with 1×105 cells/mL in concentration in PBS were prepared. Single-cell suspensions were loaded into microfluidic devices and scRNA-seq libraries were constructed following the Singleron GEXSCOPER protocol by GEXSCOPER Single-Cell RNA Library Kit (Singleron Biotechnologies).
Indivaidual libraries of each sample were then dilyted to 4nM, pooled and sequenced on Illumina novaseq 6000 with 150 bp paired end reads.
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description Singleron Matrix GEXSCOPE
Data processing The demultiplexing, barcoded processing, alignment, gene counting and aggregation were made using the Singleron Celescope software version 1.10.0 (
The doublet were removed by using Doubletfinder R package and personal checking for conserved markers.
The R package Seurat version 4.1.1 was used for normalization, clustering and reduction. Cell type was annotated based on conserved markers.
Differential gene expression analysis and enrichment analysis were performed by using MAST and clusterProfiler R package.
Assembly: GRCm38
Supplementary files format and content: Compressed .tar.gz file containing genes, barcodes and matrix for each sample
Submission date Jun 20, 2023
Last update date Mar 15, 2024
Contact name Jinhang He
Phone 15821251893
Organization name Xiamen University
Street address No.4221, South Xiang'an Road
City Xiamen
State/province Fujian
ZIP/Postal code 361104
Country China
Platform ID GPL24247
Series (2)
GSE235290 Combination therapy with oncolytic virus and adoptively transferred T cells or mRNA vaccine amplifies antitumor effects [scRNA-Seq]
GSE235291 Combination therapy with oncolytic virus and adoptively transferred T cells or mRNA vaccine amplifies antitumor effects
BioSample SAMN35813328
SRA SRX20732281

Supplementary file Size Download File type/resource
GSM7498398_PBS_P14_2.tar.gz 38.0 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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