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| Status |
Public on Mar 15, 2024 |
| Title |
P14 T cells sorted from B16-OVA tumor treated with rVSV-LCMVG, Replicate 1 |
| Sample type |
SRA |
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| Source name |
P14_T_cells
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| Organism |
Mus musculus |
| Characteristics |
cell line: P14_T_cells
treatment: P14 T cells sorted from B16-OVA tumor treated with rVSV-LCMVG
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| Extracted molecule |
total RNA |
| Extraction protocol |
The P14-T cell and OT1-T cell were sorted and stored in RNA Later Solution (Thermo Fisher Scientific) for a maximum of 24 h at 4 °C. Lung tissue was homogenized using TissueLyser II (Qiagen, Hilden, Germany) and RNA extraction was performed according to the manufacturer’s instructions (QIAamp Viral RNA Mini Kit (Qiagen)).
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
VSVgp_P14_1
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| Data processing |
Sequence reads were trimmed and adaptor removed by using fastp.
Cleanned sequence reads were mapped to GRcm38 Genome by using Hisat2
Raw count were calculated by using featurecounts
Assembly: GRcm38
Supplementary files format and content: tab-delimited txt files include raw count values for each Sample
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| Submission date |
Jun 20, 2023 |
| Last update date |
Mar 15, 2024 |
| Contact name |
Jinhang He |
| E-mail(s) |
hejinhang@stu.xmu.edu.cn
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| Phone |
15821251893
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| Organization name |
Xiamen University
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| Street address |
No.4221, South Xiang'an Road
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| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361104 |
| Country |
China |
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| Platform ID |
GPL17021 |
| Series (2) |
| GSE235289 |
Combination therapy with oncolytic virus and adoptively transferred T cells or mRNA vaccine amplifies antitumor effects [RNA-Seq] |
| GSE235291 |
Combination therapy with oncolytic virus and adoptively transferred T cells or mRNA vaccine amplifies antitumor effects |
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| Relations |
| BioSample |
SAMN35813764 |
| SRA |
SRX20732790 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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