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Sample GSM7494538 Query DataSets for GSM7494538
Status Public on Feb 28, 2024
Title Positive
Sample type protein
 
Source name Purified IgG
Organism Homo sapiens
Characteristics status: Positive for T. cruzi infection
sample type: Pool of IgG from 9 subjects
Extracted molecule protein
Extraction protocol IgG antibodies were purified from human plasma samples using Thermo Scientific™ Melon™ Gel IgG Spin Purification Kit
Label Cy3 goat anti-Human IgG
Label protocol none provided
 
Hybridization protocol Microarrray slides were deprotected in TFA EDT H20 for 3h at room temperature and blocked overnight in 0.1% BSA, 0.1% Tween-20 in PBS. After blocking, slides were incubated for 1h at RT with one of the IgG probes (100 µg/ml IgG in 0. 1% BSA, 0.1% Tween-20 in PBS), washed 3 x 20 min with 0.1% BSA, 0.1% Tween-20 in PBS and incubated for 1h at RT with Cy3- goat anti-Human IgG (1 µg/ml in 0.1% B SA, 0.1% Tween-20 in PBS). Slides were washed 3 x 20 min with 0.1% BSA in PBS, dried
Scan protocol Scanned using a laser scanner with 1 µm resolution
Description A pool of IgG from 9 subjects positive for T. cruzi infection was incubated with an overlapping peptide array of the Trypanosoma cruzi conserved proteome.
Data processing Statistics assuming normal distribution of signals from fields with no sequence (= blanks): signal-µblanks = signal from peptide minus mean of blank fields in the sector indicated.
 
Submission date Jun 15, 2023
Last update date Feb 28, 2024
Contact name Eric Dumonteil
E-mail(s) edumonte@tulane.edu
Organization name Tulane University
Department Tropical Medicine
Street address 1440 Canal st.
City New Orleans
State/province LA
ZIP/Postal code 70115
Country USA
 
Platform ID GPL33498
Series (1)
GSE235074 Trypanosoma cruzi conserved proteome microarray

Data table header descriptions
ID_REF
VALUE signal-µblanks = signal from peptide minus mean of blank fields in the sector indicated; z-score = number of blank-standard deviations the peptide signal is above the blank-mean in the sector indicated.; p-value = the probability that the signal is not significant.

Data table
ID_REF VALUE
1 35452
2 35547
3 35812
4 36079
5 36718
6 37116
7 37474
8 37903
9 37956
10 37950
11 38190
12 38213
13 38305
14 38489
15 38583
16 38761
17 38802
18 38771
19 38870
20 38901

Total number of rows: 198

Table truncated, full table size 1 Kbytes.




Supplementary file Size Download File type/resource
GSM7494538_POS_Array_Part1.txt.gz 4.3 Mb (ftp)(http) TXT
GSM7494538_POS_Array_Part2.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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