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Sample GSM7476243 Query DataSets for GSM7476243
Status Public on Jul 03, 2023
Title Input_Azin2_KO_REP2
Sample type SRA
 
Source name Adipose
Organism Mus musculus
Characteristics tissue: Adipose
cell type: Adipocyte Progenitor
genotype: Azin2-/-
Extracted molecule genomic DNA
Extraction protocol Immunoprecipitation was performed by incubating the sheared chromatin with the magna ChIP protein A/G magnetic beads and an antibody against H3K27Ac (Millipore 17- 683).
Libraries were generated using the NEBNext Ultra II DNA library Prep kit kit (Illumina Inc)
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NovaSeq 6000
 
Data processing Nextflow (v 22.02.1) [3] was used to run the nf-core/chipseq: 2.0.0 [4] workflow pipeline with the following parameters : --genome GRCm38 --macs_pvalue 0.1 --max_cpus 4 --macs_gsize 1.87e9.
Supplementary files format and content: mm10
Supplementary files format and content: bigWig and narrowPeak
 
Submission date Jun 14, 2023
Last update date Jul 03, 2023
Contact name Ismini Vasileia Alexaki
E-mail(s) VasileiaIsmini.Alexaki@ukdd.de
Organization name IKL , Carl Gustav Carus Universitätsklinikum, TU Dresden
Street address Fetscherstraße 74
City Dresden
State/province Saxony
ZIP/Postal code 01307
Country Germany
 
Platform ID GPL24247
Series (2)
GSE234890 ChIP-seq in Azin2-/- and wt adipocyte progenitors
GSE235012 Azin2-dependent transcriptional and epigenetic regulation in adipose tissue and adipocyte progenitors
Relations
BioSample SAMN35731976
SRA SRX20675672

Supplementary file Size Download File type/resource
GSM7476243_Input_Azin2_KO_REP2.bigWig 149.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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