|
| Status |
Public on Jul 03, 2023 |
| Title |
H3K27ac_Azin2_WT_REP1 |
| Sample type |
SRA |
| |
|
| Source name |
Adipose
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adipose
cell type: Adipocyte Progenitor
genotype: WT
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Immunoprecipitation was performed by incubating the sheared chromatin with the magna ChIP protein A/G magnetic beads and an antibody against H3K27Ac (Millipore 17- 683).
Libraries were generated using the NEBNext Ultra II DNA library Prep kit kit (Illumina Inc)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Nextflow (v 22.02.1) [3] was used to run the nf-core/chipseq: 2.0.0 [4] workflow pipeline with the following parameters : --genome GRCm38 --macs_pvalue 0.1 --max_cpus 4 --macs_gsize 1.87e9.
Supplementary files format and content: mm10
Supplementary files format and content: bigWig and narrowPeak
|
| |
|
| Submission date |
Jun 14, 2023 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Ismini Vasileia Alexaki |
| E-mail(s) |
VasileiaIsmini.Alexaki@ukdd.de
|
| Organization name |
IKL , Carl Gustav Carus Universitätsklinikum, TU Dresden
|
| Street address |
Fetscherstraße 74
|
| City |
Dresden |
| State/province |
Saxony |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE234890 |
ChIP-seq in Azin2-/- and wt adipocyte progenitors |
| GSE235012 |
Azin2-dependent transcriptional and epigenetic regulation in adipose tissue and adipocyte progenitors |
|
| Relations |
| BioSample |
SAMN35731980 |
| SRA |
SRX20675668 |
