|
Status |
Public on Aug 20, 2023 |
Title |
Tmem119-creERT2-cGaswt/R241E microglia whole brain mouse 3, scRNAseq |
Sample type |
SRA |
|
|
Source name |
hippocampus
|
Organism |
Mus musculus |
Characteristics |
tissue: hippocampus cell type: microglia genotype: cGasR241E
|
Treatment protocol |
Aged mice were injected intraperitoneally with 750 nmol H151 or DMSO per mouse five days a week for 8 weeks. The mice were rested for 8 weeks, and 8 weeks of treatment were repeated one more time.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Nuclei from mouse brain were extracted by homogenizing mouse brain tissues in Nuclei EZ Lysis Buffer using a douncer. Nuclei were then loaded into a Chromium Single Cell Controller. ChromiumSingle Cell 3’ Library & Gel Bead Kit v3.1
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Illumina BCL convert was used to demultiplex reads 10X Genomics Cell Ranger Single Cell Software Suite v7.0.0 was used to perform barcode processing and 3’ gene counting using 10X Genomics custom annotation of murine genome assembly mm10 Analysis was performed using Seurat in R Assembly: mm10 Supplementary files format and content: tab-seperated filtered values and matrix files
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|
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Submission date |
Jun 07, 2023 |
Last update date |
Aug 20, 2023 |
Contact name |
Andrea Ablasser |
E-mail(s) |
andrea.ablasser@epfl.ch
|
Organization name |
EPFL
|
Lab |
Ablasser
|
Street address |
route Cantonale
|
City |
Lausanne |
ZIP/Postal code |
1015 |
Country |
Switzerland |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE234421 |
cGAS/STING drives ageing-related inflammation and neurodegeneration IV |
GSE234422 |
cGAS/STING drives ageing-related inflammation and neurodegeneration |
|
Relations |
BioSample |
SAMN35668017 |
SRA |
SRX20631978 |