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Sample GSM7467270 Query DataSets for GSM7467270
Status Public on Aug 20, 2023
Title Tmem119-creERT2-cGaswt/R241E microglia whole brain mouse 3, scRNAseq
Sample type SRA
 
Source name hippocampus
Organism Mus musculus
Characteristics tissue: hippocampus
cell type: microglia
genotype: cGasR241E
Treatment protocol Aged mice were injected intraperitoneally with 750 nmol H151 or DMSO per mouse five days a week for 8 weeks. The mice were rested for 8 weeks, and 8 weeks of treatment were repeated one more time.
Extracted molecule polyA RNA
Extraction protocol Nuclei from mouse brain were extracted by homogenizing mouse brain tissues in Nuclei EZ Lysis Buffer using a douncer. Nuclei were then loaded into a Chromium Single Cell Controller.
ChromiumSingle Cell 3’ Library & Gel Bead Kit v3.1
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Illumina BCL convert was used to demultiplex reads
10X Genomics Cell Ranger Single Cell Software Suite v7.0.0 was used to perform barcode processing and 3’ gene counting using 10X Genomics custom annotation of murine genome assembly mm10
Analysis was performed using Seurat in R
Assembly: mm10
Supplementary files format and content: tab-seperated filtered values and matrix files
 
Submission date Jun 07, 2023
Last update date Aug 20, 2023
Contact name Andrea Ablasser
E-mail(s) andrea.ablasser@epfl.ch
Organization name EPFL
Lab Ablasser
Street address route Cantonale
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platform ID GPL24247
Series (2)
GSE234421 cGAS/STING drives ageing-related inflammation and neurodegeneration IV
GSE234422 cGAS/STING drives ageing-related inflammation and neurodegeneration
Relations
BioSample SAMN35668017
SRA SRX20631978

Supplementary file Size Download File type/resource
GSM7467270_S3_lox3_barcodes.tsv.gz 13.0 Kb (ftp)(http) TSV
GSM7467270_S3_lox3_features.tsv.gz 284.1 Kb (ftp)(http) TSV
GSM7467270_S3_lox3_matrix.mtx.gz 28.2 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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