|
| Status |
Public on Oct 08, 2024 |
| Title |
Post-stressed rat (S2) |
| Sample type |
SRA |
| |
|
| Source name |
Testicular tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Testicular tissue
treatment: Terrified-sound induced psychological stress
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Transfer fresh tissue to a culture dish containing HBSS buffer and wash it three time. Afterwards, remove the excess buffer and transfer the tissue to a 1.5mL low-adsorption centrifuge tube. Add 200 μL of Tissue Dissociation Mix, and use sterile ophthalmic scissors to cut the tissue to minced form. After adding 1 mL of Tissue Dissociation Mix, the minced tissue homogenate was transferred to a 15 mL low-adsorption centrifuge tube for tissue dissociation. The dissociated cell suspension was filtered through a 40um filter. And the Single-cell suspensions were prepared at a concentration of 1 × 105 cells/mL in PBS (HyClone).
Single-cell suspensions were loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPE® protocol by GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies). Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina NovaSeq with 150 bp paired end reads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Singleron MatrixTM Single cell RNA sequencing
|
| Data processing |
The demultiplexing, barcoded processing and gene counting were made using the Celescope v1.8.1
Assembly: mRatBN6.0
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jun 01, 2023 |
| Last update date |
Oct 08, 2024 |
| Contact name |
Yungang Xu |
| E-mail(s) |
yungang.xu@xjtu.edu.cn
|
| Organization name |
Xi'an Jiaotong University
|
| Street address |
Xi'an, Shaanxi Province
|
| City |
Xi'an, Shaanxi Province |
| State/province |
Shaanxi |
| ZIP/Postal code |
710049 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE233950 |
Single-cell transcriptome profiling implicates the psychological stress-induced disruption of spermatogenesis |
|
| Relations |
| BioSample |
SAMN35561907 |
| SRA |
SRX20572236 |
