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Sample GSM743698 Query DataSets for GSM743698
Status Public on Dec 25, 2011
Title CC_ADSV28F.undiff..3._8.2010 [HuGene-1_0-st]
Sample type RNA
 
Source name ADSV_28_derived_MPCs_rep3
Organism Homo sapiens
Characteristics cell type: MPC
Extracted molecule total RNA
Extraction protocol Total RNA from human cell lines or human fat was extracted with Trizol (Invitrogen) and purified via the RNeasy mini kit (Qiagen) according to the manufacturers’ instructions. RNA quality was assessed using an Aglient Bioanalyzer
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description Gene Expression
MINTY_p_Cowen_Affy1.0ST_plate_HuGene-1_0-st-v1.r4_B12_646930.CEL
CC_ADSV28F(undiff)(3)_8-2010
Data processing Data were processed using the BioConductor affy package in R. RMA background correction was applied to probe intensities followed by quantile normalization. Probe set intensities were summarized from individual probes (excluding mismatch probes) using median polished. Gene level intensities were generated by mapping probe sets to refseq annotations. Probe sets that did not map to refseq annotations were not considered in further analyses. Probe Sets mapping to the same genes were median collapsed by gene symbol. Final normalized data was generated by median centering each array after the prior processing.
probe group file: [Exon array] probe group file: HuGene-1_0-st-v1.r4.pgf/HG-U133_Plus_2.pgf
meta-probeset file: [Exon array] meta-probeset file: HuGene-1_0-st-v1.r4.mps/HG-U133_Plus_2.mps
 
Submission date Jun 16, 2011
Last update date Dec 25, 2011
Contact name Tim D Ahfeldt
E-mail(s) tim.ahfeldt@mssm.edu
Organization name Icahn School of Medicine at Mount Sinai
Department Neuroscience
Lab Tim D Ahfeldt
Street address 1425 Madison Ave
City NYC
State/province NY
ZIP/Postal code 10029
Country USA
 
Platform ID GPL6244
Series (2)
GSE30038 Programming human pluripotent stem cells into adipocytes [Affymetrix]
GSE30041 Programming human pluripotent stem cells into adipocytes

Data table header descriptions
ID_REF
VALUE Quantile normalized, median centered(array) gene level expression values from Bioconductor affy package

Data table
ID_REF VALUE
7892501 14.25496627
7892502 27.05751367
7892503 12.09512695
7892504 364.5896655
7892505 20.27787791
7892506 41.5335814
7892507 55.23418157
7892508 28.86388141
7892509 1861.826253
7892510 30.99033243
7892511 35.38698624
7892512 96.52356288
7892513 23.41549404
7892514 793.4763247
7892515 831.5813473
7892516 38.23441631
7892517 85.57909732
7892518 10.11642832
7892519 39.08164881
7892520 523.371438

Total number of rows: 32321

Table truncated, full table size 627 Kbytes.




Supplementary file Size Download File type/resource
GSM743698.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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