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Sample GSM7431486 Query DataSets for GSM7431486
Status Public on Jan 08, 2024
Title 96h_2
Sample type SRA
 
Source name H99
Organism Cryptococcus neoformans var. grubii H99
Characteristics strain: H99
genotype: WT
treatment: YPD liquid medium, 96h
Extracted molecule polyA RNA
Extraction protocol The level and integrity of RNA in each sample were evaluated using a Qubit RNA Assay Kit on a Qubit 2.0 Flurometer (Life Technologies, CA, USA) and RNA Nano 6000 Assay Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively. RNA purity was assessed using a Nano Photometer spectrophotometer (IMPLEN, CA, USA).
The transcriptome libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit (Vazyme Biotech Co., Ltd, Nanjing, China) according to the manufacturer’s instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing FastQC v0.11.5
trimmomatic v0.39
Hisat2 v2.1.0
Stringtie v1.3.3
DEseq2 v1.16.1
Assembly: Cryptococcus neoformans var. grubii H99
Supplementary files format and content: tpm TPM of all strains
 
Submission date May 27, 2023
Last update date Jan 08, 2024
Contact name hao ding
E-mail(s) dinghaov587@126.com
Organization name institute of microbiology
Street address BeiChen Road
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL33437
Series (1)
GSE233619 Fungicide-tolerant persister formation during cryptococcal pulmonary infection
Relations
BioSample SAMN35444786
SRA SRX20528767

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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