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Sample GSM7426327 Query DataSets for GSM7426327
Status Public on Jul 02, 2024
Title Middle-age, 4, spatial transcriptomics
Sample type SRA
 
Source name Whole brain
Organism Mus musculus
Characteristics cell type: Brain cells
tissue: Whole brain
strain: C57Bl/JRj
age: 9-11 month old
Extracted molecule total RNA
Extraction protocol For scRNA-seq, freshly collected brain cells from dentate gyrus of C57Bl6/JRj mice were dissociated using Neural Tissue Dissociation Kit (P) and Myelin Removal Beads II (Miltenyi Biotec). For spatial transcriptomics, mouse brains were collected and embedded in OCT.
Library was performed according to the manufacter’s instructions (single cell 3’ v3.1 protocol, spatial gene expression protocol, 10x Genomics).
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina NovaSeq 6000
 
Description 10x Genomics
Data processing Raw data was processed using the Cell Ranger (v6) and Space Ranger (v2) softwares.
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
Library strategy: Spatial Transcriptomics
 
Submission date May 24, 2023
Last update date Jul 02, 2024
Contact name Yicheng Wu
E-mail(s) yicheng.wu1987@gmail.com
Organization name University of Zurich
Department Brain Research Institute
Street address Winterthurerstrasse 190
City Zurich
ZIP/Postal code 8057
Country Switzerland
 
Platform ID GPL24247
Series (1)
GSE233363 Multimodal transcriptomic analysis of aging neurogenic niche in the mouse hippocampus
Relations
BioSample SAMN35354667
SRA SRX20509338

Supplementary file Size Download File type/resource
GSM7426327_P23360_1012_S12_barcodes.tsv.gz 16.2 Kb (ftp)(http) TSV
GSM7426327_P23360_1012_S12_features.tsv.gz 284.1 Kb (ftp)(http) TSV
GSM7426327_P23360_1012_S12_matrix.mtx.gz 51.9 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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