|
| Status |
Public on Feb 21, 2024 |
| Title |
Back epidermis KO2 |
| Sample type |
SRA |
| |
|
| Source name |
Back epidermis
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Back epidermis
genotype: Spry1flox/flox K14-Cre-ERT2
treatment: Tamoxifen
|
| Treatment protocol |
Tamoxifen (MCE) treatment was performed by oral gavage of tamoxifen 4 times/week within 4 weeks (0.1 mg/g body weight) of a 20 mg/ml solution in corn oil.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. 0.4 ug of total RNA was used for the construction of sequencing libraries；
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
(1)Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence; (2) paired-end clean reads were aligned to the Mus Musculus(GRCm38/mm10) using hisat2 (v2.0.5)；(3)featureCounts (v1.5.0-p3) was used to count the reads numbers mapped to each gene.
And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Assembly: GRCm38
Supplementary files format and content: Tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
May 19, 2023 |
| Last update date |
Feb 21, 2024 |
| Contact name |
Yingzhe Cui |
| E-mail(s) |
12018453@zju.edu.cn
|
| Phone |
19883158324
|
| Organization name |
Second Affiliated Hospital, Zhejiang University School of Medicine
|
| Street address |
Jiefang road
|
| City |
Hangzhou |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE232912 |
RNA-seq analysis comparing Spry1flox/flox mice back epidermis to the back epidermis derived from Spry1 epidermis specific knockout mice |
|
| Relations |
| BioSample |
SAMN35215847 |
| SRA |
SRX20438540 |
