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Status |
Public on May 18, 2024 |
Title |
AML-12 cells, overexpression, GM47544-2 |
Sample type |
SRA |
|
|
Source name |
AML-12
|
Organism |
Mus musculus |
Characteristics |
cell line: AML-12 cell type: hepatocyte genotype: GM47544 overexpressed
|
Growth protocol |
AML-12 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using Trizol reagent. A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Raw reads in fastq format were processed to remove contaminating adapter sequences using Trimmomatics Quality distribution plot and base content distribution were generated by FASTQC RNA-seq reads were aligned to the mm10 genome using STAR. Read count extraction were performed using HTSeq. Assembly: mm10 Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
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|
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Submission date |
May 19, 2023 |
Last update date |
May 18, 2024 |
Contact name |
Qianqian Xiao |
Organization name |
Tongji Hospital
|
Department |
Departments of Internal Medicine
|
Lab |
Division of Cardiology
|
Street address |
1095# Jiefang Ave., Wuhan 430030 People’s Rep. of China
|
City |
Wuhan |
ZIP/Postal code |
430030 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE232866 |
Ubiquitination of hepatic APOC3 by GM47544 prevents hypertriglyceridemia and ameliorates atherosclerosis |
|
Relations |
BioSample |
SAMN35177437 |
SRA |
SRX20435425 |