|Public on Dec 01, 2011
|development stage: St. 18
tissue: tail section
sample origin/type: single embryo
|Total RNA was collected by homogenization in Invitrogen TRIzol reagent and further purified using an Qiagen RNeasy micro kit.
|Biotinylated cRNA were prepared according to the standard 2-cycle Affymetrix protocol from 100ng of total RNA (Expression Analysis Technical Manual, Affymetrix).
|Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on Xenopus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|GeneChips were scanned using the standard Affymetrix protocol.
|Gene expression data from tail
|The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
|Jun 07, 2011
|Last update date
|May 10, 2017
|1550 Fourth St, 382
|Gene expression profile for X.laevis embryo head vs.tail
|Identification of putative targets of Nkx2-5 in Xenopus laevis using gene expression analysis and cross-species.