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Sample GSM738186 Query DataSets for GSM738186
Status Public on Jan 20, 2012
Title 30do mouse spermatids replicate 1
Sample type RNA
 
Source name 30do mouse spermatids
Organism Mus musculus
Characteristics background strain: BALB/c
gender: male
age: 30 days old
organ: testes
Treatment protocol Balb/c 30do male mice were killed by CO2, testes were exised dy the scissors
Growth protocol Balb/c male mice were kept in cages till they reach the age of 30 days old
Extracted molecule total RNA
Extraction protocol Testis tubules from wild type Balb/c mice were placed in 1-2 ml of ice-cold PBS-GL buffer (PBS supplemented with 5.6mM D-glucose, 5.4mM DL-lactate, 0.5mM DTT, 0.2mM PMSF, and protease inhibitor cocktail (Roche)) and dissected with scissors followed by homogenisation with 3-4 strokes in a Dounce homogeniser (pestle type A, cell integrity monitored by microscopy). The cellular mixture was filtered through a 70┬Ám cell strainer to obtain a single cell suspension
Label biotin
Label protocol 200ng of total RNA using the Ambion WT kit
 
Hybridization protocol We employed standard Affymetrix hybridisation protocol (45C for 17 hours at 60 rpm)
Scan protocol Arrays were scanned using a GCS 3000 7G scanner
Description Gene expression data from 30 do mouse spermatids
Data processing CEL files were generated Affymetrix GeneChip Command Console (AGCC) software. Further analysis performed in R (see manuscript)
 
Submission date Jun 07, 2011
Last update date Jan 20, 2012
Contact name Rohan Williams
E-mail(s) rohan.williams@anu.edu.au
Organization name ANU
Street address Garran Rd ACTON
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
 
Platform ID GPL6246
Series (2)
GSE29781 Expression data from 30do mouse spermatid [Affymetrix]
GSE29916 Functional studies of a H2A.Bbd-like histone variant in mouse spermatogenesis

Data table header descriptions
ID_REF
VALUE RMA expression summaries from R/Bioconductor package 'affy'.

Data table
ID_REF VALUE
10338001 12.7379
10338002 6.40685
10338003 11.28032
10338004 10.6025
10338005 3.25542
10338006 3.4507
10338007 3.75997
10338008 4.31803
10338009 7.47628
10338010 3.2929
10338011 6.04523
10338012 3.36076
10338013 3.13681
10338014 3.1904
10338015 3.14477
10338016 7.09687
10338017 13.36235
10338018 6.82509
10338019 5.54889
10338020 7.68117

Total number of rows: 35556

Table truncated, full table size 590 Kbytes.




Supplementary file Size Download File type/resource
GSM738186.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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