NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM73556 Query DataSets for GSM73556
Status Public on Sep 20, 2006
Title LOF_stage11_replicate3
Sample type RNA
 
Channel 1
Source name stage11 embryo wt
Organism Drosophila melanogaster
Characteristics Oregon R wildtype embryos
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage11 embryo mutant
Organism Drosophila melanogaster
Characteristics gcm mutant embryos automated sorted
Extracted molecule polyA RNA
Label Cy5
 
 
Description gcmN7-4 mutant flies were balanced with either Kr-GFP (for stages 10 to 13) or Ubi-GFP (for stages 14 to 16) balancer chromosomes. Eggs were collected as described above, transferred into PBS buffer supplemented with 0.3% Tween 20 and homozygous mutant embryos were automatically sorted with the CopasTM Select embryo sorter by their lack of GFP-expression. For wt control we used OregonR wildtype embryos that were treated the same way like the mutant embryos.Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide.
Data processing Genepix 5.0/MChips software
 
Submission date Sep 08, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F Pixels
B Pixels
Circularity
Sum of Medians (632/532)
Sum of Means (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F Pixels B Pixels Circularity Sum of Medians (632/532) Sum of Means (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 VALUE
1 01_61_03_12 5250 5310 80 16190 15018 3590 922 922 925 327 100 100 0 59839 54227 14134 2534 2534 2705 929 100 100 44 0.266 0.273 0.268 0.275 1.134 0.266 0.973 52 226 100 72573 65789 15268 57305 14096 51693 -1.908
2 01_61_05_12 5360 5310 100 880 918 325 879 879 891 310 17 5 0 2812 2820 777 2260 2260 2331 871 31 8 0 0.002 0.070 0.317 0.386 4.943 0.297 0.395 80 279 100 553 599 1 552 39 560 -9.109
3 01_61_07_12 5500 5310 100 786 796 255 795 795 800 262 15 5 0 1948 1938 575 2019 2019 1979 714 8 0 0 0.127 -0.012 0.431 0.370 3.843 0.053 0.014 80 264 100 -80 -80 -9 -71 1 -81 -2.980
4 01_61_09_12 5630 5310 100 869 880 343 856 856 883 374 15 3 0 2245 2224 725 2018 2018 2027 724 26 2 0 0.057 0.117 0.472 0.543 4.217 0.111 0.047 80 286 100 240 230 13 227 24 206 -4.126
5 01_61_11_12 5750 5310 70 6418 6087 1366 1220 1220 1365 602 100 100 0 8035 7831 1506 1926 1926 1960 679 100 100 0 0.851 0.824 0.810 0.818 1.234 0.770 0.856 32 166 100 11307 10772 5198 6109 4867 5905 -0.233
6 01_61_13_12 5880 5330 90 65535 52747 17445 1523 1523 1736 823 100 100 53 65535 52501 19160 2174 2174 2172 792 100 100 59 1.010 1.018 1.010 1.044 1.235 0.986 0.962 52 227 100 127373 101551 64012 63361 51224 50327 0.015
7 01_61_15_12 6010 5330 100 65535 55853 16586 1616 1616 1938 990 100 100 70 65535 53363 19526 2418 2418 2413 895 100 100 65 1.013 1.065 1.013 1.148 1.509 1.012 0.933 80 258 100 127036 105182 63919 63117 54237 50945 0.018
8 01_61_01_24 6140 5330 90 59296 50821 17717 1375 1375 1604 896 100 100 36 65535 52042 19526 2497 2497 2476 872 100 100 51 0.919 0.998 1.018 1.063 1.445 0.962 0.945 52 232 100 120959 98991 57921 63038 49446 49545 -0.122
9 01_61_03_24 6300 5320 100 903 947 251 923 923 1017 566 2 0 0 2066 2180 818 1961 1961 2005 795 22 6 0 -0.190 0.110 0.419 0.435 4.818 0.224 0.071 80 316 100 85 243 -20 105 24 219 0
10 01_61_05_24 6440 5320 100 734 766 253 746 746 774 285 16 2 0 2178 2128 673 1945 1945 1956 687 22 3 0 -0.052 0.109 0.354 0.362 7.780 116.596 0.000 80 291 100 221 203 -12 233 20 183 0
11 01_61_07_24 6570 5320 100 838 838 255 747 747 767 296 18 3 0 2321 2425 782 2172 2172 2198 715 23 7 0 0.611 0.360 0.364 0.349 3.954 0.032 0.006 80 300 100 240 344 91 149 91 253 -0.711
12 01_61_09_24 6710 5320 90 1792 1801 374 773 773 807 306 100 82 0 3870 3859 734 2178 2178 2226 705 88 63 0 0.602 0.612 0.599 0.669 2.043 0.369 0.293 52 289 100 2711 2709 1019 1692 1028 1681 -0.732
13 01_61_11_24 6860 5320 90 1720 1716 419 775 775 809 310 98 76 0 3762 3772 918 1997 1997 2006 675 90 63 0 0.535 0.530 0.517 0.591 2.269 0.307 0.299 52 253 100 2710 2716 945 1765 941 1775 -0.901
14 01_61_13_24 6970 5330 100 864 881 301 768 768 779 283 31 8 0 2166 2175 724 1895 1895 1908 651 30 10 0 0.354 0.404 0.414 0.354 4.578 0.063 0.013 80 260 100 367 393 96 271 113 280 -1.497
15 01_61_15_24 7110 5330 100 1010 1089 457 766 766 806 280 46 31 0 2386 2339 761 1978 1978 2028 766 31 5 0 0.598 0.895 0.591 0.543 4.735 0.176 0.091 80 262 100 652 684 244 408 323 361 -0.742
16 01_62_01_12 7240 5330 100 778 807 274 816 816 823 303 12 2 0 2233 2257 704 2159 2159 2236 836 16 1 0 -0.514 -0.092 0.185 0.352 6.013 65.016 0.001 80 254 100 36 89 -38 74 -9 98 0
17 01_62_03_12 7370 5330 100 793 812 245 761 761 776 304 17 0 0 2276 2271 679 2040 2040 2070 761 26 3 0 0.136 0.221 0.332 0.340 5.614 0.051 0.011 80 276 100 268 282 32 236 51 231 -2.883
18 01_62_05_12 7510 5330 100 744 744 259 744 744 761 285 15 1 0 2088 2083 600 2187 2187 2211 711 7 0 0 0.000 0.000 0.817 0.680 6.530 0.016 0.001 80 290 100 -99 -104 0 -99 0 -104 0
19 01_62_07_12 7640 5340 100 734 775 270 721 721 769 319 11 3 0 2185 2088 667 2310 2310 2324 690 6 1 0 -0.104 -0.243 0.289 0.316 5.990 36.563 0.002 80 260 100 -112 -168 13 -125 54 -222 0
20 01_62_09_12 7770 5340 100 803 805 273 727 727 777 318 20 1 0 2313 2369 761 2501 2501 2554 813 13 0 0 -0.404 -0.591 0.351 0.327 3.924 77.551 0.001 80 301 100 -112 -54 76 -188 78 -132 0

Total number of rows: 47616

Table truncated, full table size 9300 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap