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Sample GSM735542 Query DataSets for GSM735542
Status Public on Jul 13, 2011
Title Alzheimer's Disease serum (early stage AD vs. NDC) sample 23
Sample type other
 
Source name AD23
Organism Homo sapiens
Characteristics age: 84
gender: Male
mini mental state exam (mmse) score: 22
sample type: serum
disease state: Alzheimer's Disease
Extracted molecule other
Extraction protocol All serum (PD vs. BC) (PD vs. AD) samples were purchased from commercial biofluid repositiories, drawn and stored under proper conditions, and collected and used with IRB approval.
Label Alexa Fluor 647
Label protocol standard Invitrogen protocol
 
Hybridization protocol Probing and analysis of microarrays was performed with strict adherence to suggested manufacturer protocols. (See Invitrogen and Molecular Devices online.)
Scan protocol Protein microarrays were scanned using a Genepix 4000B microarray scanner at 635nm using default parameters and a pixel size of 5um.
Data processing The GPR files were imported into Invitrogen's Prospector v5.2 microarray analysis software. Individual serum (PD vs. BC) (PD vs. AD) results were characterized into disease and control groups and then linearly normalized for group-to-group analysis of differential expression.
 
Submission date Jun 01, 2011
Last update date Jul 13, 2011
Contact name Robert G Nagele
E-mail(s) nagelero@rowan.edu
Phone 856-566-6083
Organization name UMDNJ-SOM
Department NJISA
Lab Nagele
Street address 2 Medical Center Drive
City Stratford
State/province NJ
ZIP/Postal code 08084
Country USA
 
Platform ID GPL13669
Series (1)
GSE29676 Diagnosis of Alzheimer's Disease Based on Disease-Specific Autoantibody Profiles
Relations
Reanalysis of GSM734859

Data table header descriptions
ID_REF
VALUE Normalized fluorescence intensities are presented in Relative Fluorescent Units (RFUs).

Data table
ID_REF VALUE
1 1.499408169
2 4457.910276
3 4926.72739
4 4403.284819
5 2992.930817
6 17569.41813
7 97101.40339
8 68732.81338
9 91809.25242
10 93975.7298
11 88995.26626
12 11446.88198
13 3210.242451
14 97179.34521
15 75923.16365
16 97265.70425
17 84273.76678
18 70268.28938
19 84784.31211
20 15139.95751

Total number of rows: 9480

Table truncated, full table size 155 Kbytes.




Supplementary file Size Download File type/resource
GSM735542_PA25476_-_AD23.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table
Processed data are available on Series record

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