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Sample GSM73539 Query DataSets for GSM73539
Status Public on Sep 20, 2006
Title LOF_stage10_replicate1
Sample type RNA
 
Channel 1
Source name stage10 embryo mutant
Organism Drosophila melanogaster
Characteristics gcm mutant embryos automated sorted
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage10 embryo wt
Organism Drosophila melanogaster
Characteristics Oregon R wildtype embryos
Extracted molecule polyA RNA
Label Cy5
 
 
Description gcmN7-4 mutant flies were balanced with either Kr-GFP (for stages 10 to 13) or Ubi-GFP (for stages 14 to 16) balancer chromosomes. Eggs were collected as described above, transferred into PBS buffer supplemented with 0.3% Tween 20 and homozygous mutant embryos were automatically sorted with the CopasTM Select embryo sorter by their lack of GFP-expression. For wt control we used OregonR wildtype embryos that were treated the same way like the mutant embryos.Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide.
Data processing Genepix 5.0/MChips software
 
Submission date Sep 08, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R? (632/532)
F Pixels
B Pixels
Circularity
Sum of Medians (632/532)
Sum of Means (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R? (632/532) F Pixels B Pixels Circularity Sum of Medians (632/532) Sum of Means (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 VALUE
1 01_61_03_12 3530 3720 100 6344 6167 2635 1371 1371 1428 498 96 93 0 36136 33176 19429 428 428 555 492 100 95 10 0.139 0.146 0.147 0.170 2.443 0.136 0.887 80 385 100 40680 37542 4973 35707 4795 32747 -2.844
2 01_61_05_12 3630 3740 100 1627 1655 616 1472 1472 1549 535 25 6 0 671 1488 1847 466 466 621 542 32 26 0 0.759 0.180 1.299 1.447 8.277 0.148 0.801 80 292 100 359 1204 155 204 183 1021 -0.397
3 01_61_07_12 3740 3800 50 2656 2883 690 1353 1353 1373 451 100 83 0 1943 1954 181 416 416 427 185 100 100 0 0.853 0.994 0.849 0.915 2.103 1.263 0.458 12 47 100 2829 3067 1302 1527 1529 1538 -0.229
4 01_61_09_12 3900 3740 100 1325 1396 437 1254 1254 1309 465 22 3 0 218 239 175 279 279 296 171 12 5 0 -1.173 -3.544 2.129 2.869 5.352 124.408 0.000 80 413 100 10 101 70 -60 141 -40 0
5 01_61_11_12 4070 3740 70 2526 2575 613 1290 1290 1342 456 87 75 0 2784 2810 728 323 323 335 173 100 100 0 0.502 0.517 0.493 0.455 2.446 0.506 0.430 32 189 100 3697 3771 1236 2461 1284 2487 -0.993
6 01_61_13_12 4190 3740 90 11935 12011 4853 1454 1454 1519 529 100 100 0 24074 23428 10445 430 430 455 273 100 100 0 0.443 0.459 0.470 0.459 1.558 0.450 0.947 52 255 100 34124 33554 10480 23644 10556 22998 -1.174
7 01_61_15_12 4320 3760 80 17131 21426 13706 1553 1553 1606 520 100 100 0 30035 33498 20199 547 547 610 298 100 100 26 0.528 0.603 0.601 0.590 1.850 0.620 0.942 52 161 100 45065 52822 15577 29488 19872 32950 -0.921
8 01_61_01_24 4440 3740 100 18174 20849 13892 1389 1389 1434 455 100 100 0 33863 32843 21830 435 435 497 342 100 100 30 0.502 0.600 0.630 0.632 1.713 0.608 0.963 80 305 100 50211 51868 16784 33427 19460 32408 -0.994
9 01_61_03_24 4570 3770 70 2261 2188 421 1330 1330 1429 523 75 37 0 1654 1632 478 382 382 404 204 96 96 0 0.731 0.685 0.759 0.673 3.437 0.765 0.190 32 150 100 2202 2107 930 1272 857 1250 -0.451
10 01_61_05_24 4710 3750 100 1238 1271 470 1401 1401 1403 532 8 1 0 298 320 174 321 321 376 267 6 1 0 7.058 146.810 3.457 3.097 4.213 7.651 0.053 80 336 100 -186 -130 -163 -23 -130 0 2.819
11 01_61_07_24 4840 3750 100 1302 1349 442 1312 1312 1335 499 12 2 0 314 324 148 325 325 395 269 3 0 0 0.949 -41.765 2.433 2.615 6.148 67.222 0.001 80 279 100 -20 37 -10 -10 37 0 -0.075
12 01_61_09_24 4980 3750 100 1214 1267 385 1316 1316 1333 472 10 0 0 474 493 225 309 309 315 143 52 27 0 -0.612 -0.263 1.629 1.295 5.449 0.147 0.023 80 262 100 64 136 -101 165 -48 184 0
13 01_61_11_24 5110 3750 100 1230 1301 509 1237 1237 1292 430 20 10 0 473 480 200 318 318 327 152 53 21 0 -0.043 0.394 1.760 1.340 7.312 0.013 0.000 80 262 100 149 226 -6 155 64 162 0
14 01_61_13_24 5250 3750 100 1376 1414 526 1261 1261 1311 563 17 2 0 411 502 465 365 365 1013 4285 0 0 0 2.507 1.118 2.538 3.236 6.881 0.095 0.238 80 262 100 161 288 115 46 152 136 1.326
15 01_61_15_24 5380 3750 100 1445 1676 850 1320 1320 1362 557 32 11 0 999 2976 5666 363 363 1012 4285 12 10 0 0.196 0.136 0.501 0.528 5.625 0.058 0.281 80 262 100 759 2968 124 635 355 2613 -2.348
16 01_62_01_12 5520 3750 100 1393 1428 485 1309 1309 1327 431 18 5 0 324 328 140 336 336 340 150 13 2 0 -7.302 -14.906 3.500 4.486 5.847 22.827 0.016 80 262 100 73 111 84 -11 119 -8 0
17 01_62_03_12 5650 3750 100 1346 1335 403 1308 1308 1318 416 15 2 0 321 335 153 353 353 361 156 16 1 0 -1.195 -1.446 2.239 2.681 3.507 16.090 0.021 80 273 100 6 8 38 -32 26 -18 0
18 01_62_05_12 5790 3750 100 1244 1234 367 1289 1289 1308 444 12 0 0 369 377 136 370 370 384 186 8 0 0 50.624 -7.752 3.208 2.850 5.244 27.701 0.005 80 326 100 -44 -48 -44 0 -55 7 5.662
19 01_62_07_12 5920 3750 100 1347 1392 484 1276 1276 1306 454 23 7 0 363 380 172 364 364 380 194 17 2 0 -79.733 7.242 2.430 3.140 5.241 0.057 0.004 80 308 100 70 131 70 0 115 16 0
20 01_62_09_12 6050 3750 100 1304 1294 428 1214 1214 1266 464 21 2 0 373 357 163 361 361 372 163 16 1 0 7.788 -17.972 2.718 3.193 7.146 14.907 0.015 80 280 100 100 75 89 11 79 -4 2.961

Total number of rows: 47616

Table truncated, full table size 9191 Kbytes.




Supplementary data files not provided
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