|
| Status |
Public on Jul 13, 2011 |
| Title |
Alzheimer's Disease serum (AD vs. NDC) sample 50 |
| Sample type |
other |
| |
|
| Source name |
AD50
|
| Organism |
Homo sapiens |
| Characteristics |
age: 77
gender: Male
mini mental state exam (mmse) score: 18
sample type: serum
disease state: Alzheimer's Disease
|
| Extracted molecule |
other |
| Extraction protocol |
All serum (PD vs. BC) (PD vs. AD) samples were purchased from commercial biofluid repositiories, drawn and stored under proper conditions, and collected and used with IRB approval.
|
| Label |
Alexa Fluor 647
|
| Label protocol |
standard Invitrogen protocol
|
| |
|
| Hybridization protocol |
Probing and analysis of microarrays was performed with strict adherence to suggested manufacturer protocols. (See Invitrogen and Molecular Devices online.)
|
| Scan protocol |
Protein microarrays were scanned using a Genepix 4000B microarray scanner at 635nm using default parameters and a pixel size of 5um.
|
| Data processing |
The GPR files were imported into Invitrogen's Prospector v5.2 microarray analysis software. Individual serum (PD vs. BC) (PD vs. AD) results were characterized into disease and control groups and then linearly normalized for group-to-group analysis of differential expression.
|
| |
|
| Submission date |
Jun 01, 2011 |
| Last update date |
Jul 13, 2011 |
| Contact name |
Robert G Nagele |
| E-mail(s) |
nagelero@rowan.edu
|
| Phone |
856-566-6083
|
| Organization name |
UMDNJ-SOM
|
| Department |
NJISA
|
| Lab |
Nagele
|
| Street address |
2 Medical Center Drive
|
| City |
Stratford |
| State/province |
NJ |
| ZIP/Postal code |
08084 |
| Country |
USA |
| |
|
| Platform ID |
GPL13669 |
| Series (1) |
| GSE29676 |
Diagnosis of Alzheimer's Disease Based on Disease-Specific Autoantibody Profiles |
|
| Relations |
| Reanalysis of |
GSM734851 |
