GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM733656 Query DataSets for GSM733656
Status Public on Jun 02, 2011
Title Bernstein_K562_H3K27ac
Sample type SRA
Source name K562
Organism Homo sapiens
Characteristics biomaterial_type: immortalized cell line
line: K562
lab: Broad
lab description: Bernstein - Broad Institute
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell organism: human
cell description: leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell lineage: mesoderm
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000052
labversion: Illumina_GA2e and Illumina_GA2x
replicate: 1,2
softwareversion: ScriptureVPaperR3
Biomaterial provider ATCC
Growth protocol K562_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol For extraction protocol details see:
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer II
Data processing For data processing details see:
Submission date May 27, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9115
Series (2)
GSE29611 Histone Modifications by ChIP-seq from ENCODE/Broad Institute
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Reanalyzed by GSM936088
SRA SRX067407
BioSample SAMN00622078
Named Annotation GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdSig.bigWig

Supplementary file Size Download File type/resource
GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdAlnRep1.bam 612.5 Mb (ftp)(http) BAM
GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdAlnRep2.bam 377.0 Mb (ftp)(http) BAM
GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdPk.broadPeak.gz 932.5 Kb (ftp)(http) BROADPEAK
GSM733656_hg19_wgEncodeBroadHistoneK562H3k27acStdSig.bigWig 292.5 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap