|
Status |
Public on Jun 02, 2011 |
Title |
Bernstein_NHLF_H3K27ac |
Sample type |
SRA |
|
|
Source name |
NHLF
|
Organism |
Homo sapiens |
Characteristics |
donor_id: ENCDO156MSV,ENCDO269AAA biomaterial_type: primary cell cell_type: fibroblast of lung lab: Broad lab description: Bernstein - Broad Institute datatype: ChipSeq datatype description: Chromatin IP Sequencing cell organism: human cell description: lung fibroblasts cell karyotype: normal cell lineage: endoderm antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac antibody: H3K27ac antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases. antibody vendorname: abcam antibody vendorid: ab4729 treatment: None treatment description: No special treatment or protocol applies control: std control description: Standard input signal for most experiments. controlid: wgEncodeEH000105 replicate: 1,2 softwareversion: ScriptureVPaperR3
|
Biomaterial provider |
Lonza
|
Growth protocol |
NHLF_Bernstein_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
For extraction protocol details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer II |
|
|
Data processing |
For data processing details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
|
|
Submission date |
May 27, 2011 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9115 |
Series (2) |
GSE29611 |
Histone Modifications by ChIP-seq from ENCODE/Broad Institute |
GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
Relations |
Reanalyzed by |
GSM936086 |
SRA |
SRX067397 |
BioSample |
SAMN00622068 |
Named Annotation |
GSM733646_hg19_wgEncodeBroadHistoneNhlfH3k27acStdSig.bigWig |