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Sample GSM73329 Query DataSets for GSM73329
Status Public on Sep 20, 2006
Title GOF_stage15_replicate1
Sample type RNA
 
Channel 1
Source name stage15 embryo ectopic expression
Organism Drosophila melanogaster
Characteristics ectopic expression of glial cells missing with the Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage15 embryo wt
Organism Drosophila melanogaster
Characteristics pooled wildtypcontrol from Bloomington stock B5446 and Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy5
 
 
Description Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide. For wt control we pooled mRNA from UAS-gcm stock B5446 and Gal4-line MZ1060 in equal amounts.
Data processing Genepix5.0/MChips software
 
Submission date Sep 06, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians (632/532)
Sum of Means (632/532)
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians (632/532) Sum of Means (632/532) F Pixels B Pixels VALUE
1 01_61_03_12 4620 5280 90 14307 13447 3171 1484 1499 306 100 100 0 21790 19999 5720 653 713 509 100 100 0 0.607 0.618 0.612 0.627 1.182 0.602 0.972 12823 21137 11963 19346 33960 31309 52 303 -0.720
2 01_61_05_12 4730 5280 100 1434 1444 291 1510 1514 322 8 0 0 623 619 392 535 627 514 11 2 0 -0.864 -0.786 0.890 0.810 3.461 0.648 0.627 -76 88 -66 84 12 18 80 269 0
3 01_61_07_12 4860 5280 100 1360 1375 208 1370 1390 284 7 1 0 378 416 340 416 436 330 16 2 0 0.263 100.000.000 0.775 1.017 5.308 0.052 0.001 -10 -38 5 0 -48 5 80 253 -1.927
4 01_61_09_12 4990 5280 100 1475 1464 260 1342 1370 271 20 6 0 440 499 352 474 473 346 18 2 0 -3.912 4.880 0.746 0.808 2.791 10.949 0.003 133 -34 122 25 99 147 80 352 0
5 01_61_11_12 5140 5280 80 2052 2096 414 1380 1439 386 78 40 0 1156 1162 464 541 877 1380 5 0 0 1.093 1.153 1.135 1.093 3.014 0.226 0.217 672 615 716 621 1287 1337 52 209 0.128
6 01_61_13_12 5260 5290 80 6657 6996 2804 1468 1532 407 100 100 0 5942 6198 2893 401 823 1487 98 82 0 0.936 0.954 0.964 0.948 1.273 0.922 0.853 5189 5541 5528 5797 10730 11325 52 184 -0.095
7 01_61_15_12 5390 5280 80 9399 9544 3037 1445 1473 307 100 100 0 9942 9857 3428 375 392 310 100 100 0 0.831 0.854 0.848 0.851 1.175 0.848 0.954 7954 9567 8099 9482 17521 17581 52 198 -0.267
8 01_61_01_24 5510 5290 80 9067 8914 2761 1440 1444 282 100 100 0 8674 8372 3204 341 381 305 100 100 0 0.915 0.931 0.928 0.963 1.252 0.910 0.953 7627 8333 7474 8031 15960 15505 52 205 -0.128
9 01_61_03_24 5660 5290 100 1386 1387 268 1340 1365 274 20 3 0 306 333 294 414 440 338 8 0 0 -0.426 -0.580 0.660 0.669 4.422 0.237 0.005 46 -108 47 -81 -62 -34 80 300 0
10 01_61_05_24 5790 5290 100 1498 1507 263 1342 1378 304 23 5 0 464 514 401 461 487 383 20 5 0 52.000 3.113 0.778 0.825 7.451 8.051 0.004 156 3 165 53 159 218 80 309 5.700
11 01_61_07_24 5930 5290 100 1422 1411 279 1356 1366 278 21 2 0 654 607 389 570 591 382 15 2 0 0.786 1.486 0.851 0.705 3.604 0.023 0.000 66 84 55 37 150 92 80 318 -0.347
12 01_61_09_24 6040 5290 50 2181 2160 233 1410 1437 298 100 75 0 1466 1277 427 570 607 399 83 58 0 0.860 1.061 0.898 0.997 1.815 0.392 0.122 771 896 750 707 1667 1457 12 84 -0.218
13 01_61_11_24 6190 5290 100 1535 1564 320 1370 1386 261 43 11 0 483 523 380 429 459 358 25 7 0 3.056 2.064 0.757 0.721 3.392 0.203 0.009 165 54 194 94 219 288 80 330 1.612
14 01_61_13_24 6330 5290 100 1540 1552 295 1340 1376 261 38 13 0 790 823 498 405 440 333 55 27 0 0.519 0.507 0.515 0.513 3.865 0.193 0.033 200 385 212 418 585 630 80 262 -0.946
15 01_61_15_24 6460 5290 100 1480 1493 318 1364 1396 269 26 11 0 686 742 469 498 530 356 37 15 0 0.617 0.529 0.769 0.706 4.078 0.231 0.043 116 188 129 244 304 373 80 256 -0.697
16 01_62_01_12 6590 5300 100 1436 1447 279 1408 1426 279 22 2 0 748 763 437 690 661 372 18 10 0 0.483 0.534 0.582 0.634 3.419 0.002 0.000 28 58 39 73 86 112 80 254 -1.050
17 01_62_03_12 6730 5300 100 1385 1429 271 1417 1438 282 16 1 0 715 757 398 712 714 406 20 5 0 -10.667 0.267 0.816 0.757 2.865 0.033 0.001 -32 3 12 45 -29 57 80 273 0
18 01_62_05_12 6860 5300 100 1304 1359 266 1399 1424 268 10 3 0 811 801 334 834 837 385 7 1 0 4.130 1.212 0.751 0.608 5.409 0.089 0.001 -95 -23 -40 -33 -118 -73 80 267 2.046
19 01_62_07_12 6990 5300 100 1351 1398 267 1341 1372 240 28 6 0 870 851 400 891 879 369 13 3 0 -0.476 -1.425 0.533 0.603 7.381 8.567 0.009 10 -21 57 -40 -11 17 80 253 0
20 01_62_09_12 7130 5300 100 1376 1407 308 1370 1387 272 21 8 0 879 833 422 891 866 404 10 2 0 -0.500 -0.638 0.555 0.565 4.762 0.108 0.007 6 -12 37 -58 -6 -21 80 245 0

Total number of rows: 47616

Table truncated, full table size 8576 Kbytes.




Supplementary data files not provided

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