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Sample GSM73325 Query DataSets for GSM73325
Status Public on Sep 20, 2006
Title GOF_stage11_replicate4
Sample type RNA
 
Channel 1
Source name stage11 embryo wt
Organism Drosophila melanogaster
Characteristics pooled wildtypcontrol from Bloomington stock B5446 and Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage11 embryo ectopic expression
Organism Drosophila melanogaster
Characteristics ectopic expression of glial cells missing with the Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy5
 
 
Description Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide. For wt control we pooled mRNA from UAS-gcm stock B5446 and Gal4-line MZ1060 in equal amounts.
Data processing Genepix5.0/MChips software
 
Submission date Sep 06, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians (632/532)
Sum of Means (632/532)
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians (632/532) Sum of Means (632/532) F Pixels B Pixels VALUE
1 01_61_03_12 5000 5230 90 35687 33541 9228 1930 1990 583 100 100 0 49729 46611 17830 1125 1206 797 100 100 15 0.695 0.695 0.705 0.757 1.401 0.649 0.938 33757 48604 31611 45486 82361 77097 52 314 -0.525
2 01_61_05_12 5110 5230 100 2170 2190 556 1958 2079 975 10 0 0 1092 1077 656 1153 1273 811 8 0 0 -3.475 -3.053 0.581 0.667 4.359 0.476 0.770 212 -61 232 -76 151 156 80 284 0
3 01_61_07_12 5240 5230 100 1841 1878 458 1727 1864 935 3 0 0 1034 1066 605 923 964 563 27 5 0 1.027 1.056 0.972 1.031 3.965 4.912 0.045 114 111 151 143 225 294 80 268 0.038
4 01_61_09_12 5380 5230 100 1718 1743 456 1751 1750 406 15 6 0 1102 1141 582 1067 1068 579 13 5 0 -0.943 -0.108 0.904 0.979 6.740 59.013 0.000 -33 35 -8 74 2 66 80 312 0
5 01_61_11_12 5500 5240 80 3217 3137 703 1823 1826 421 90 73 0 2860 2928 990 1244 1246 610 84 63 0 0.863 0.780 0.841 0.879 2.670 0.627 0.321 1394 1616 1314 1684 3010 2998 52 190 -0.213
6 01_61_13_12 5630 5250 80 11600 12164 5163 1909 1952 532 100 100 0 13667 13748 6805 1146 1144 612 100 98 0 0.774 0.814 0.820 0.830 1.486 0.765 0.874 9691 12521 10255 12602 22212 22857 52 181 -0.370
7 01_61_15_12 5750 5260 90 25025 24342 7712 2005 2090 629 100 100 0 29286 28318 11722 1358 1414 727 100 100 0 0.824 0.829 0.786 0.916 1.619 0.779 0.913 23020 27928 22337 26960 50948 49297 52 208 -0.279
8 01_61_01_24 5890 5230 80 17997 16788 5900 2040 2056 525 100 100 0 23596 20447 9624 1414 1420 707 100 100 0 0.719 0.775 0.750 0.845 1.432 0.713 0.914 15957 22182 14748 19033 38139 33781 52 247 -0.476
9 01_61_03_24 6040 5240 100 1938 1938 503 2003 2029 490 13 2 0 1547 1607 566 1306 1342 657 32 2 0 -0.270 -0.216 0.723 0.715 6.875 4.458 0.017 -65 241 -65 301 176 236 80 311 0
10 01_61_05_24 6180 5240 100 2250 2253 490 2186 2186 481 16 5 0 1782 1788 642 1554 1607 696 21 5 0 0.281 0.286 0.520 0.348 5.334 7.627 0.006 64 228 67 234 292 301 80 308 -1.831
11 01_61_07_24 6310 5240 100 2127 2148 551 2093 2089 497 21 5 0 2224 2175 559 1800 1777 695 31 2 0 0.080 0.147 0.656 0.790 5.526 17.001 0.001 34 424 55 375 458 430 80 324 -3.644
12 01_61_09_24 6450 5250 60 3617 3827 1071 1905 1883 426 93 84 0 3930 4134 1398 1643 1647 662 87 84 0 0.749 0.772 0.690 0.751 2.306 0.744 0.528 1712 2287 1922 2491 3999 4413 32 152 -0.417
13 01_61_11_24 6590 5250 70 4005 3758 1026 1703 1742 431 96 84 0 3961 3876 1137 1354 1305 626 93 90 0 0.883 0.815 0.824 0.978 2.793 0.764 0.567 2302 2607 2055 2522 4909 4577 32 172 -0.180
14 01_61_13_24 6710 5250 100 1810 1817 431 1698 1739 466 17 3 0 1474 1461 682 1152 1152 598 31 10 0 0.348 0.385 0.639 0.593 4.677 0.158 0.010 112 322 119 309 434 428 80 371 -1.523
15 01_61_15_24 6880 5240 60 4812 4800 1758 1782 1823 452 96 93 0 3958 4300 1951 1049 1027 520 96 87 0 1.042 0.928 0.985 0.926 1.542 0.910 0.774 3030 2909 3018 3251 5939 6269 32 132 0.059
16 01_62_01_12 6980 5250 100 1860 1839 462 1724 1759 436 21 6 0 1164 1143 612 1042 1069 552 22 1 0 1.115 1.139 0.702 0.693 4.770 0.153 0.008 136 122 115 101 258 216 80 282 0.157
17 01_62_03_12 7120 5250 100 1620 1684 468 1797 1824 463 13 2 0 1095 1146 611 1126 1190 574 20 1 0 5.710 -5.650 1.028 1.091 3.721 0.100 0.003 -177 -31 -113 20 -208 -93 80 276 2.513
18 01_62_05_12 7250 5250 100 1720 1764 453 1811 1858 475 12 2 0 1235 1281 626 1126 1164 618 25 2 0 -0.835 -0.303 0.588 0.604 4.397 14.113 0.001 -91 109 -47 155 18 108 80 277 0
19 01_62_07_12 7380 5250 100 1853 1858 442 1814 1850 460 15 2 0 1003 1108 655 1274 1286 638 12 1 0 -0.144 -0.265 0.493 0.550 4.076 0.048 0.002 39 -271 44 -166 -232 -122 80 267 0
20 01_62_09_12 7520 5260 100 1860 1847 488 1794 1844 514 15 1 0 1138 1268 618 1260 1278 630 15 1 0 -0.541 6.625 0.815 0.744 4.169 0.137 0.011 66 -122 53 8 -56 61 80 257 0

Total number of rows: 47616

Table truncated, full table size 8838 Kbytes.




Supplementary data files not provided

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