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Sample GSM73324 Query DataSets for GSM73324
Status Public on Sep 20, 2006
Title GOF_stage16_replicate4
Sample type RNA
 
Channel 1
Source name stage16 embryo wt
Organism Drosophila melanogaster
Characteristics pooled wildtypcontrol from Bloomington stock B5446 and Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage16 embryo ectopic expression
Organism Drosophila melanogaster
Characteristics ectopic expression of glial cells missing with the Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy5
 
 
Description Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide. For wt control we pooled mRNA from UAS-gcm stock B5446 and Gal4-line MZ1060 in equal amounts.
Data processing Genepix5.0/MChips software
 
Submission date Sep 06, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians (632/532)
Sum of Means (632/532)
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians (632/532) Sum of Means (632/532) F Pixels B Pixels VALUE
1 01_61_03_12 4930 5180 100 1417 1399 454 1209 1232 357 37 11 0 5010 5019 2921 364 402 355 95 91 0 0.045 0.041 0.080 0.076 4.147 0.037 0.056 208 4646 190 4655 4854 4845 80 351 -4.474
2 01_61_05_12 5030 5190 100 1338 1320 349 1261 1293 354 17 2 0 305 351 273 458 523 386 6 1 0 -0.503 -0.551 0.812 0.731 4.619 0.138 0.013 77 -153 59 -107 -76 -48 80 241 0
3 01_61_07_12 5160 5190 100 1308 1313 374 1207 1236 371 23 5 0 344 430 374 474 510 389 15 3 0 -0.777 -2.409 0.770 0.781 4.633 8.310 0.000 101 -130 106 -44 -29 62 80 287 0
4 01_61_09_12 5300 5190 100 1226 1282 328 1229 1266 391 16 2 0 451 483 368 476 479 319 22 7 0 0.120 7.571 0.956 0.869 6.413 0.976 0.002 -3 -25 53 7 -28 60 80 308 -3.059
5 01_61_11_12 5420 5220 60 2542 2495 524 1333 1355 363 90 81 0 1001 999 422 432 438 328 71 46 0 2.125 2.049 1.654 1.933 2.066 3.284 0.127 1209 569 1162 567 1778 1729 32 109 1.087
6 01_61_13_12 5560 5210 80 12247 11843 3690 1351 1425 442 100 100 0 5768 5597 2016 439 465 322 100 96 0 2.045 2.034 2.105 2.116 1.268 1.994 0.950 10896 5329 10492 5158 16225 15650 52 197 1.032
7 01_61_15_12 5670 5210 90 29594 28703 11544 1557 1646 579 100 100 0 17177 15599 6545 707 777 497 100 100 0 1.702 1.823 1.839 1.891 1.265 1.824 0.970 28037 16470 27146 14892 44507 42038 52 215 0.767
8 01_61_01_24 5810 5210 80 14972 15542 4366 1426 1494 488 100 100 0 7896 7893 2557 684 687 439 100 100 0 1.878 1.958 1.938 2.020 1.219 1.904 0.966 13546 7212 14116 7209 20758 21325 52 212 0.909
9 01_61_03_24 5960 5200 100 1328 1369 391 1285 1320 364 21 6 0 517 545 291 653 665 377 6 0 0 -0.316 -0.778 0.940 1.159 3.919 2.880 0.008 43 -136 84 -108 -93 -24 80 311 0
10 01_61_05_24 6100 5200 100 1361 1352 367 1279 1288 340 21 7 0 716 696 370 664 679 365 17 2 0 1.577 2.281 0.735 0.704 4.113 4.095 0.001 82 52 73 32 134 105 80 308 0.657
11 01_61_07_24 6230 5200 100 1330 1357 365 1300 1369 477 12 1 0 611 694 358 789 826 489 5 0 0 -0.169 -0.600 1.015 0.827 4.731 0.731 0.021 30 -178 57 -95 -148 -38 80 280 0
12 01_61_09_24 6370 5200 100 1471 1539 520 1322 1365 491 28 5 0 811 806 370 759 803 480 12 0 0 2.865 4.617 1.541 1.435 4.240 1.179 0.107 149 52 217 47 201 264 80 268 1.519
13 01_61_11_24 6500 5210 100 1466 1562 525 1266 1273 358 37 23 0 889 910 371 658 676 357 38 8 0 0.866 1.175 1.704 1.410 2.999 2.928 0.028 200 231 296 252 431 548 80 242 -0.208
14 01_61_13_24 6630 5210 100 1332 1369 369 1315 1301 374 21 2 0 727 771 355 743 735 368 17 5 0 -1.062 1.929 0.801 0.893 4.883 1.443 0.005 17 -16 54 28 1 82 80 262 0
15 01_61_15_24 6770 5210 100 1334 1389 382 1314 1328 388 17 5 0 754 758 329 712 693 370 17 1 0 0.476 1.630 0.808 1.022 6.230 2.118 0.002 20 42 75 46 62 121 80 262 -1.071
16 01_62_01_12 6900 5210 100 1294 1296 426 1359 1351 342 18 3 0 798 827 398 685 694 378 26 7 0 -0.575 -0.444 1.243 1.005 3.914 0.594 0.016 -65 113 -63 142 48 79 80 250 0
17 01_62_03_12 7030 5210 100 1331 1333 360 1359 1355 362 12 1 0 762 777 344 822 854 428 7 1 0 0.467 0.578 0.960 0.925 5.017 0.662 0.003 -28 -60 -26 -45 -88 -71 80 323 -1.099
18 01_62_05_12 7170 5260 70 1976 1915 440 1495 1536 454 50 15 0 2321 2483 1008 1144 1207 559 71 56 0 0.409 0.314 0.265 0.337 3.281 0.268 0.086 481 1177 420 1339 1658 1759 32 168 -1.290
19 01_62_07_12 7300 5210 100 1311 1368 408 1411 1481 501 12 0 0 945 922 443 994 1067 557 7 1 0 2.041 0.597 0.998 1.249 3.808 0.414 0.175 -100 -49 -43 -72 -149 -115 80 357 1.029
20 01_62_09_12 7440 5220 100 1238 1254 407 1308 1350 439 13 1 0 993 1013 458 1010 1024 433 15 5 0 4.118 -18.000 1.453 1.222 5.996 1.960 0.004 -70 -17 -54 3 -87 -51 80 254 2.042

Total number of rows: 47616

Table truncated, full table size 8554 Kbytes.




Supplementary data files not provided

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