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Sample GSM73315 Query DataSets for GSM73315
Status Public on Sep 20, 2006
Title GOF_stage11_replicate3
Sample type RNA
 
Channel 1
Source name Stage11 embryo wt
Organism Drosophila melanogaster
Characteristics pooled wildtypcontrol from Bloomington stock B5446 and Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage11 embryo ectopic expression
Organism Drosophila melanogaster
Characteristics ectopic expression of glial cells missing with the Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy5
 
 
Description Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide. For wt control we pooled mRNA from UAS-gcm stock B5446 and Gal4-line MZ1060 in equal amounts.
Data processing Genepix5.0/MChips software
 
Submission date Sep 06, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians (632/532)
Sum of Means (632/532)
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532) Not normalized!

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians (632/532) Sum of Means (632/532) F Pixels B Pixels VALUE
1 01_61_03_12 4430 5400 90 20242 19722 3996 2629 2677 697 100 100 0 48256 44731 12790 1584 1690 866 100 100 0 0.377 0.396 0.395 0.408 1.300 0.377 0.932 17613 46672 17093 43147 64285 60240 52 321 -1.407
2 01_61_05_12 4580 5400 100 2693 2685 598 2489 2542 652 21 3 0 2139 2038 753 1942 2000 875 10 1 0 1.036 2.042 1.079 1.210 3.746 0.037 0.000 204 197 196 96 401 292 80 329 0.051
3 01_61_07_12 4720 5400 100 2722 2736 691 2524 2685 977 12 2 0 1945 1942 754 1941 2498 3189 0 0 0 49.500 212.000 0.794 1.007 4.104 0.359 0.320 198 4 212 1 202 213 80 276 5.629
4 01_61_09_12 4850 5400 100 2614 2661 631 2654 2809 989 3 1 0 1927 2001 862 1665 2261 3190 1 0 0 -0.153 0.021 0.552 0.726 3.876 0.231 0.551 -40 262 7 336 222 343 80 278 0
5 01_61_11_12 4970 5420 70 9233 9025 2275 2979 3102 871 100 100 0 5691 5749 1400 1677 1677 671 100 96 0 1.558 1.485 1.549 1.478 1.571 1.472 0.736 6254 4014 6046 4072 10268 10118 32 137 0.640
6 01_61_13_12 5100 5410 80 65535 57066 14558 3593 4064 1856 100 100 67 56995 46445 21672 2038 2143 987 100 100 40 1.127 1.204 1.127 1.392 1.547 1.086 0.898 61942 54957 53473 44407 116899 97880 52 196 0.172
7 01_61_15_12 5220 5410 90 65535 61968 8549 4301 5066 3771 100 100 82 65535 56262 16453 2570 2729 1614 100 100 67 0.973 1.074 0.973 1.144 1.423 0.995 0.893 61234 62965 57667 53692 124199 111359 52 191 -0.039
8 01_61_01_24 5350 5410 100 65535 49441 21277 3239 3697 2593 100 96 57 40164 40396 24141 2278 2365 1232 97 95 38 1.644 1.212 1.161 1.487 1.749 1.086 0.905 62296 37886 46202 38118 100182 84320 80 338 0.717
9 01_61_03_24 5550 5430 60 2993 3025 702 2826 2864 697 31 3 0 3128 3552 1312 1579 1646 668 81 65 0 0.108 0.101 0.394 0.299 2.802 0.138 0.051 167 1549 199 1973 1716 2172 32 98 -3.211
10 01_61_05_24 5650 5400 100 2645 2649 588 2565 2593 583 18 3 0 1706 1717 778 1681 1728 721 16 6 0 3.200 2.333 0.611 0.659 6.328 25.242 0.001 80 25 84 36 105 120 80 274 1.678
11 01_61_07_24 5780 5400 100 2665 2815 650 2671 2659 643 20 6 0 1913 2042 650 1903 1946 716 17 5 0 -0.600 1.036 0.764 1.061 6.644 0.986 0.015 -6 10 144 139 4 283 80 262 0
12 01_61_09_24 5920 5400 100 2659 2777 791 2766 2801 616 20 7 0 1927 2038 812 2069 2078 758 17 2 0 0.754 -0.355 1.090 1.177 3.482 0.732 0.053 -107 -142 11 -31 -249 -20 80 262 -0.407
13 01_61_11_24 6050 5400 100 2683 2738 662 2707 2752 625 17 7 0 1885 1819 738 2147 2163 784 7 0 0 0.092 -0.095 0.959 0.851 7.491 0.160 0.007 -24 -262 31 -328 -286 -297 80 262 -3.442
14 01_61_13_24 6190 5400 100 2560 2633 624 2601 2668 604 18 5 0 2005 2237 928 2155 2199 722 23 10 0 0.273 0.390 0.707 0.626 4.793 0.308 0.037 -41 -150 32 82 -191 114 80 262 -1.873
15 01_61_15_24 6320 5400 100 2820 2827 583 2588 2677 648 23 5 0 1979 2049 731 2270 2292 744 15 1 0 -0.797 -1.081 0.903 0.817 4.119 0.495 0.015 232 -291 239 -221 -59 18 80 250 0
16 01_62_01_12 6450 5400 100 2588 2666 571 2647 2671 615 16 2 0 1919 1963 756 2402 2394 772 3 0 0 0.122 -0.043 0.746 0.594 4.338 0.189 0.008 -59 -483 19 -439 -542 -420 80 262 -3.035
17 01_62_03_12 6590 5400 100 2435 2447 565 2608 2654 594 11 0 0 2055 2075 733 2179 2197 751 11 1 0 1.395 1.548 0.562 0.539 5.288 24.459 0.000 -173 -124 -161 -104 -297 -265 80 262 0.480
18 01_62_05_12 6720 5400 100 2583 2740 685 2588 2672 630 23 6 0 1868 1935 736 2165 2246 780 7 2 0 0.017 -0.661 0.721 0.645 7.956 0.138 0.004 -5 -297 152 -230 -302 -78 80 250 -5.878
19 01_62_07_12 6850 5400 100 2602 2587 568 2580 2628 638 15 2 0 1589 1734 775 2104 2194 733 8 1 0 -0.043 -0.019 0.474 0.621 6.114 0.021 0.000 22 -515 7 -370 -493 -363 80 262 0
20 01_62_09_12 6990 5400 100 2691 2722 597 2700 2693 614 17 2 0 1865 1870 697 2164 2213 708 10 1 0 0.030 -0.075 0.709 0.642 5.136 5.955 0.003 -9 -299 22 -294 -308 -272 80 262 -5.059

Total number of rows: 47616

Table truncated, full table size 8672 Kbytes.




Supplementary data files not provided

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