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Sample GSM73314 Query DataSets for GSM73314
Status Public on Sep 20, 2006
Title GOF_Stage12_replicate2
Sample type RNA
 
Channel 1
Source name stage12 embryo ectopic expression
Organism Drosophila melanogaster
Characteristics ectopic expression of glial cells missing with the Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy3
 
Channel 2
Source name stage12 embryo wt
Organism Drosophila melanogaster
Characteristics pooled wildtypcontrol from Bloomington stock B5446 and Gal4-driver MZ1060
Extracted molecule polyA RNA
Label Cy5
 
 
Description Cy5/Cy3 labeled RNA was co-hybridized onto a QMT Amino (Quantifoil) glass slide. For wt control we pooled mRNA from UAS-gcm stock B5446 and Gal4-line MZ1060 in equal amounts.
Data processing Genepix5.0/MChips software
 
Submission date Sep 06, 2005
Last update date Sep 19, 2006
Contact name Angela Becker
E-mail(s) angibeck@gmx.de
Organization name University of Mainz
Department Institute of Genetics
Lab AG Technau
Street address Becherweg 32
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platform ID GPL517
Series (1)
GSE3257 Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

Data table header descriptions
ID_REF
MChips_ID MChips_ID for inhouse datawarehouse system
X X-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Y Y-coordinate of the center of the feature indicator associated with the feature,where (0,0) is the top left of the image
Dia. Diameter in µm of the feature- indicator
F632 Median Median pixel intensity of feature for Cy5 channel
F632 Mean Mean pixel intensity of feature for Cy5 channel
F632 SD
B632 Median
B632 Mean
B632 SD
% > B632+1SD
% > B632+2SD
F632 % Sat.
F532 Median Median pixel intensity of feature for Cy3 channel
F532 Mean Mean pixel intensity of feature for Cy3 channel
F532 SD
B532 Median
B532 Mean
B532 SD
% > B532+1SD
% > B532+2SD
F532 % Sat.
Ratio of Medians (632/532)
Ratio of Means (632/532)
Median of Ratios (632/532)
Mean of Ratios (632/532)
Ratios SD (632/532)
Rgn Ratio (632/532)
Rgn R² (632/532)
F632 Median - B632
F532 Median - B532
F632 Mean - B632
F532 Mean - B532
Sum of Medians (632/532)
Sum of Means (632/532)
F Pixels
B Pixels
VALUE log2 ratio (log2 of ratio of Ratio of Means (632/532)

Data table
ID_REF MChips_ID X Y Dia. F632 Median F632 Mean F632 SD B632 Median B632 Mean B632 SD % > B632+1SD % > B632+2SD F632 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (632/532) Ratio of Means (632/532) Median of Ratios (632/532) Mean of Ratios (632/532) Ratios SD (632/532) Rgn Ratio (632/532) Rgn R² (632/532) F632 Median - B632 F532 Median - B532 F632 Mean - B632 F532 Mean - B532 Sum of Medians (632/532) Sum of Means (632/532) F Pixels B Pixels VALUE
1 01_61_03_12 4950 5300 90 26451 25480 6021 2425 2454 600 100 100 0 65535 58857 14282 3154 3272 1228 100 100 76 0.385 0.414 0.425 0.419 1.412 0.371 0.839 24026 62381 23055 55703 86407 78758 52 308 -1.377
2 01_61_05_12 5090 5300 100 2246 2315 605 2410 2412 572 15 2 0 3119 3006 952 2948 3068 1061 12 1 0 -0.959 -1.638 0.807 0.712 3.888 64.879 0.000 -164 171 -95 58 7 -37 80 317 0
3 01_61_07_12 5230 5300 100 2174 2275 492 2328 2318 541 15 2 0 2471 2503 1093 2746 2736 932 16 1 0 0.560 0.218 0.378 0.348 4.190 65.920 0.000 -154 -275 -53 -243 -429 -296 80 276 -0.837
4 01_61_09_12 5360 5300 100 2477 2512 575 2426 2449 636 17 1 0 2671 2731 1008 2881 2919 1029 16 3 0 -0.243 -0.573 0.836 0.922 7.641 0.089 0.007 51 -210 86 -150 -159 -64 80 281 0
5 01_61_11_12 5470 5340 70 9744 10515 2664 2774 2851 878 100 100 0 12419 12625 2366 3493 3421 1071 100 100 0 0.781 0.848 0.814 0.826 1.384 0.848 0.731 6970 8926 7741 9132 15896 16873 32 78 -0.357
6 01_61_13_12 5580 5320 100 65535 48262 21752 2996 3203 1138 100 100 52 65535 44961 24803 3625 3591 1097 93 92 51 1.010 1.095 1.010 1.407 2.147 1.008 0.853 62539 61910 45266 41336 124449 86602 80 236 0.014
7 01_61_15_12 5710 5330 100 65535 61229 10114 3867 4154 1488 100 100 77 65535 54503 20322 3977 4106 1449 100 97 72 1.002 1.135 1.002 1.373 1.996 0.994 0.809 61668 61558 57362 50526 123226 107888 80 194 0.003
8 01_61_01_24 5840 5330 100 65535 57154 14228 3558 3935 1499 100 100 68 65535 53685 20659 3879 4008 1477 98 96 67 1.005 1.076 1.005 1.285 2.091 0.993 0.825 61977 61656 53596 49806 123633 103402 80 279 0.007
9 01_61_03_24 6030 5310 100 2506 2576 603 2654 2831 1050 3 0 0 3697 3716 967 3332 3429 1132 23 5 0 -0.405 -0.203 0.802 0.631 7.118 0.076 0.005 -148 365 -78 384 217 306 80 337 0
10 01_61_05_24 6160 5310 100 2612 2634 534 2434 2416 575 21 5 0 3479 3391 1071 3332 3316 1033 13 5 0 1.211 3.390 0.375 0.412 5.885 53.619 0.001 178 147 200 59 325 259 80 324 0.276
11 01_61_07_24 6300 5310 100 2402 2471 667 2379 2425 589 20 7 0 3462 3417 927 3393 3422 991 12 2 0 0.333 3.833 0.951 0.772 3.968 89.535 0.000 23 69 92 24 92 116 80 276 -1.586
12 01_61_09_24 6430 5310 100 2701 2673 623 2427 2487 564 22 8 0 3637 3512 1077 3156 3186 1041 25 2 0 0.570 0.691 0.537 0.554 6.440 0.071 0.006 274 481 246 356 755 602 80 265 -0.811
13 01_61_11_24 6570 5320 100 2680 2659 650 2419 2425 555 31 12 0 3565 3772 1184 3216 3127 970 36 15 0 0.748 0.432 0.541 0.715 3.347 0.113 0.022 261 349 240 556 610 796 80 256 -0.419
14 01_61_13_24 6700 5320 100 2552 2548 613 2430 2463 570 23 7 0 3253 3310 1111 3158 3091 960 23 5 0 1.284 0.776 0.472 0.443 7.618 104.868 0.000 122 95 118 152 217 270 80 254 0.361
15 01_61_15_24 6830 5320 100 2763 2746 626 2439 2432 558 31 11 0 3597 3563 946 3037 3038 957 32 7 0 0.579 0.584 0.577 0.535 4.373 0.177 0.039 324 560 307 526 884 833 80 262 -0.788
16 01_62_01_12 6970 5320 100 2300 2358 523 2368 2360 615 11 3 0 3007 2977 995 2987 3026 1014 15 3 0 -3.400 1.000 0.603 0.591 5.434 0.038 0.001 -68 20 -10 -10 -48 -20 80 262 0
17 01_62_03_12 7100 5320 100 2235 2312 555 2323 2394 639 18 1 0 3249 3270 995 3502 3527 1183 11 0 0 0.348 0.047 0.626 0.537 6.744 0.003 0.000 -88 -253 -11 -232 -341 -243 80 268 -1.523
18 01_62_05_12 7230 5320 100 2582 2577 514 2492 2489 599 15 2 0 3740 3693 1292 3716 3769 1524 12 0 0 3.750 -3.696 0.292 0.332 4.543 0.008 0.000 90 24 85 -23 114 62 80 319 1.907
19 01_62_07_12 7370 5320 100 2398 2436 571 2425 2445 598 18 2 0 2718 3046 1006 2874 2933 1139 20 1 0 0.173 0.064 0.778 0.634 4.741 1.256.126 0.000 -27 -156 11 172 -183 183 80 310 -2.531
20 01_62_09_12 7500 5330 100 2223 2302 578 2464 2458 560 8 1 0 3057 2829 1001 2912 2934 1134 11 0 0 -1.662 1.952 0.475 0.449 4.096 0.059 0.008 -241 145 -162 -83 -96 -245 80 269 0

Total number of rows: 47616

Table truncated, full table size 8784 Kbytes.




Supplementary data files not provided

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