|
| Status |
Public on Jan 31, 2012 |
| Title |
Healthy exocervix CEXO-33 |
| Sample type |
RNA |
| |
|
| Source name |
Healthy female exocervix
|
| Organism |
Homo sapiens |
| Characteristics |
clinical group: Control
haplogroup: none
tissue: Healthy female exocervix
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ssDNA were hybridized for 16 hr at 45C and 60 rpm on Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 protocol FS450_0007
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) with GeneChip Scanner 3000
|
| Description |
Gene expression data from Healthy female exocervix
|
| Data processing |
Microarrays were normalized using the RMA algorithm (Robust Multichip Average) in the Affymetrix Expression Console. The analysis was done at Gene Level Default: RMA-Sketch
|
| |
|
| Submission date |
May 26, 2011 |
| Last update date |
Feb 26, 2020 |
| Contact name |
Mariano Guardado-Estrada |
| E-mail(s) |
jos_mariano@hotmail.com
|
| Phone |
+525527892000
|
| Fax |
+525527892000
|
| Organization name |
Hospital General de México O.D.
|
| Department |
Medicina Genómica
|
| Street address |
Dr. Bálmis 148 Col. Doctores
|
| City |
Mexico City |
| State/province |
Cuauhtémoc |
| ZIP/Postal code |
06726 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29570 |
The mtDNA Amerindian Haplogroup B2 enhances the risk for Cervical Cancer of HPV: de-regulation of mitochondrial genes may be involved. |
|
| Relations |
| Reanalyzed by |
GSM1277843 |
| Reanalyzed by |
GSE145976 |
