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Sample GSM727277 Query DataSets for GSM727277
Status Public on Feb 27, 2012
Title LA+HYP_2
Sample type RNA
Source name MCF7 cells
Organism Homo sapiens
Characteristics cell line: MCF7
growth conditions: media with 10 mM lactic acid (pH 6.7) in hypoxia (1% pO2)
Treatment protocol MCF7 cells were exposed to control condition (ambient air ~21% O2, no lactate and neutral pH), lactic acidosis (ambient air, 10 mM Lactate and pH 6.7), hypoxia (1% pO2, no lactate and neutral pH) and the combined lactic acidosis and hypoxia (1% pO2, 10 mM Lactate and pH 6.7) condition for 24 hours.
Growth protocol MCF7 breast cancer cells were cultured in DMEM (GIBCO-11995) supplemented with 10% fetal bovine serum and 1× antibiotics (penicillin, 10000UI/ml and streptomycin, 10000UI/ml).
Extracted molecule total RNA
Extraction protocol RNA were extracted by miRVana kits (Ambion) with standard protocol.
Label biotin
Label protocol Affymetrix standard protocol
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description gene expression data from MCF7 cells
Data processing Expression Console (Affymetrix) was used to generate the RMA file. Cluster 3.0 was used for hierarhical clustering.
Submission date May 19, 2011
Last update date Feb 27, 2012
Contact name Xiaohu Tang
Organization name Duke University
Department Department of Molecular Genetics and Microbiology
Street address CIEMAS Rm 2133, 101 Science Dr.
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
Platform ID GPL571
Series (1)
GSE29406 The genomic analysis of the interaction between the lactic acidosis and hypoxia response

Data table header descriptions
VALUE RMA normalized log2 data

Data table
1007_s_at 10.96453
1053_at 8.918776
117_at 5.454521
121_at 7.847581
1255_g_at 3.634857
1294_at 4.869905
1316_at 4.993046
1320_at 4.96672
1405_i_at 4.898922
1431_at 3.651644
1438_at 6.184122
1487_at 7.893671
1494_f_at 4.890602
1598_g_at 7.679665
160020_at 6.649313
1729_at 8.05922
1773_at 6.222085
177_at 5.371412
179_at 8.466219
1861_at 7.296203

Total number of rows: 22277

Table truncated, full table size 433 Kbytes.

Supplementary file Size Download File type/resource
GSM727277.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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