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Sample GSM727276 Query DataSets for GSM727276
Status Public on Feb 27, 2012
Title LA+HYP_1
Sample type RNA
 
Source name MCF7 cells
Organism Homo sapiens
Characteristics cell line: MCF7
growth conditions: media with 10 mM lactic acid (pH 6.7) in hypoxia (1% pO2)
Treatment protocol MCF7 cells were exposed to control condition (ambient air ~21% O2, no lactate and neutral pH), lactic acidosis (ambient air, 10 mM Lactate and pH 6.7), hypoxia (1% pO2, no lactate and neutral pH) and the combined lactic acidosis and hypoxia (1% pO2, 10 mM Lactate and pH 6.7) condition for 24 hours.
Growth protocol MCF7 breast cancer cells were cultured in DMEM (GIBCO-11995) supplemented with 10% fetal bovine serum and 1× antibiotics (penicillin, 10000UI/ml and streptomycin, 10000UI/ml).
Extracted molecule total RNA
Extraction protocol RNA were extracted by miRVana kits (Ambion) with standard protocol.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description gene expression data from MCF7 cells
Data processing Expression Console (Affymetrix) was used to generate the RMA file. Cluster 3.0 was used for hierarhical clustering.
 
Submission date May 19, 2011
Last update date Feb 27, 2012
Contact name Xiaohu Tang
E-mail(s) xt2@duke.edu
Organization name Duke University
Department Department of Molecular Genetics and Microbiology
Street address CIEMAS Rm 2133, 101 Science Dr.
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL571
Series (1)
GSE29406 The genomic analysis of the interaction between the lactic acidosis and hypoxia response

Data table header descriptions
ID_REF
VALUE RMA normalized log2 data

Data table
ID_REF VALUE
1007_s_at 10.956531
1053_at 8.873094
117_at 5.66927
121_at 8.100645
1255_g_at 3.549116
1294_at 4.996313
1316_at 5.359748
1320_at 4.886968
1405_i_at 4.699312
1431_at 3.774569
1438_at 6.259557
1487_at 7.876877
1494_f_at 4.900944
1598_g_at 7.782679
160020_at 6.86903
1729_at 7.994456
1773_at 6.057879
177_at 5.220046
179_at 8.56396
1861_at 7.087929

Total number of rows: 22277

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM727276.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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