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Sample GSM727275 Query DataSets for GSM727275
Status Public on Feb 27, 2012
Title HYP_3
Sample type RNA
 
Source name MCF7 cells
Organism Homo sapiens
Characteristics cell line: MCF7
growth conditions: control media in hypoxia (1% pO2)
Treatment protocol MCF7 cells were exposed to control condition (ambient air ~21% O2, no lactate and neutral pH), lactic acidosis (ambient air, 10 mM Lactate and pH 6.7), hypoxia (1% pO2, no lactate and neutral pH) and the combined lactic acidosis and hypoxia (1% pO2, 10 mM Lactate and pH 6.7) condition for 24 hours.
Growth protocol MCF7 breast cancer cells were cultured in DMEM (GIBCO-11995) supplemented with 10% fetal bovine serum and 1× antibiotics (penicillin, 10000UI/ml and streptomycin, 10000UI/ml).
Extracted molecule total RNA
Extraction protocol RNA were extracted by miRVana kits (Ambion) with standard protocol.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description gene expression data from MCF7 cells
Data processing Expression Console (Affymetrix) was used to generate the RMA file. Cluster 3.0 was used for hierarhical clustering.
 
Submission date May 19, 2011
Last update date Feb 27, 2012
Contact name Xiaohu Tang
E-mail(s) xt2@duke.edu
Organization name Duke University
Department Department of Molecular Genetics and Microbiology
Street address CIEMAS Rm 2133, 101 Science Dr.
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL571
Series (1)
GSE29406 The genomic analysis of the interaction between the lactic acidosis and hypoxia response

Data table header descriptions
ID_REF
VALUE RMA normalized log2 data

Data table
ID_REF VALUE
1007_s_at 11.256496
1053_at 7.825378
117_at 5.12299
121_at 7.871631
1255_g_at 3.50197
1294_at 4.856909
1316_at 5.120705
1320_at 4.879698
1405_i_at 5.507882
1431_at 3.50019
1438_at 6.501589
1487_at 7.857294
1494_f_at 4.861055
1598_g_at 7.712797
160020_at 6.81969
1729_at 8.758157
1773_at 6.472753
177_at 5.866123
179_at 8.42746
1861_at 7.157128

Total number of rows: 22277

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM727275.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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