|
Status |
Public on Feb 27, 2012 |
Title |
LA_1 |
Sample type |
RNA |
|
|
Source name |
MCF7 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 growth conditions: media with 10 mM lactic acid (pH 6.7)
|
Treatment protocol |
MCF7 cells were exposed to control condition (ambient air ~21% O2, no lactate and neutral pH), lactic acidosis (ambient air, 10 mM Lactate and pH 6.7), hypoxia (1% pO2, no lactate and neutral pH) and the combined lactic acidosis and hypoxia (1% pO2, 10 mM Lactate and pH 6.7) condition for 24 hours.
|
Growth protocol |
MCF7 breast cancer cells were cultured in DMEM (GIBCO-11995) supplemented with 10% fetal bovine serum and 1× antibiotics (penicillin, 10000UI/ml and streptomycin, 10000UI/ml).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA were extracted by miRVana kits (Ambion) with standard protocol.
|
Label |
biotin
|
Label protocol |
Affymetrix standard protocol
|
|
|
Hybridization protocol |
Affymetrix standard protocol
|
Scan protocol |
Affymetrix standard protocol
|
Description |
gene expression data from MCF7 cells
|
Data processing |
Expression Console (Affymetrix) was used to generate the RMA file. Cluster 3.0 was used for hierarhical clustering.
|
|
|
Submission date |
May 19, 2011 |
Last update date |
Feb 27, 2012 |
Contact name |
Xiaohu Tang |
E-mail(s) |
xt2@duke.edu
|
Organization name |
Duke University
|
Department |
Department of Molecular Genetics and Microbiology
|
Street address |
CIEMAS Rm 2133, 101 Science Dr.
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27708 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE29406 |
The genomic analysis of the interaction between the lactic acidosis and hypoxia response |
|