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Sample GSM727204 Query DataSets for GSM727204
Status Public on May 07, 2012
Title FLASH siRNA 10 hrs flash.3.10.jcp
Sample type RNA
 
Source name SW480
Organism Homo sapiens
Characteristics time point: 10 hrs
cell line: SW480
treatment: FLASH siRNA
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
Label Cy3
Label protocol Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
 
Hybridization protocol Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65┬║ C for 17 hours.
Scan protocol Agilent G2565BA
Description aneuploid, colon adenocarcinoma cell line
Data processing log2 transformation of signal intensity, quantile normalization
 
Submission date May 19, 2011
Last update date May 07, 2012
Contact name Frank Kramer
E-mail(s) frank.kramer@med.uni-goettingen.de
Organization name University Medical Center G├Âttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
 
Platform ID GPL4133
Series (1)
GSE29405 Systems-wide RNAi analysis of CASP8AP2 / FLASH

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal intensity

Data table
ID_REF VALUE
1 17.102227
2 5.228214
3 5.421488
4 5.344588
5 5.382613
6 5.382613
7 5.421488
8 5.382613
9 5.421488
10 5.344588
11 5.421488
12 11.540575
13 5.887617
14 9.377562
15 5.773599
16 13.854548
17 5.93979
18 8.879467
19 14.984261
20 6.054022

Total number of rows: 45015

Table truncated, full table size 655 Kbytes.




Supplementary file Size Download File type/resource
GSM727204_US45102909_251485048562_S01_GE1_105_Dec08_1_2.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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